Mononuclear cell infiltration and eosinophil granule proteins in the conjunctiva of patients with ocular cicatricial pemphigoid
| Primary monoclonal antibodies | |||
|---|---|---|---|
| Antibody | Dilution | Specificity | Source |
| Mononuclear cell infiltrate: | |||
| Anti-CD45 | 1/20 | Common leucocyte antigen | Dako, Hamburg, Germany |
| Anti-CD3 | 1/20 | T cell lineage, thymocytes | Dako, Hamburg, Germany |
| Anti-HLA-DR | 1/50 | HLA-DR (class II histocompatability antigen) | Dako, Hamburg, Germany |
| Eosinophil granule proteins: | |||
| EG1 | 1/200 | ECP (stored and secreted) | Kabi Pharmacia, Uppsala, Sweden |
| EG2 | 1/200 | ECP (secreted), EPX/EDN | Kabi Pharmacia, Uppsala, Sweden |
| BMK-13 | 1/50 | Major basic protein | Biodesign, Dunn, Asbach, Germany |
ECP = eosinophil cationic protein; EPX = eosinophil protein X syn; EDN = eosinophil derived neurotoxin.