Summary of light microscopic (LM) and transmission electron microscopic (TEM) findings of seven corneas examined
| No | LM: location of deposits | TEM: location and appearance | Size of particles | Remarks |
|---|---|---|---|---|
| 1 | Subepithelial, anterior half of stroma, highest accumulation in the mid stroma | Intracellular, clusters in cytoplasm surrounded by fragments of limiting mebranes | black: 40–68 nm grey: 30–51 nm | Trumps’ fixative |
| 2 | Subepithelial and anterior half of stroma | Intracellular, heavily loaded cytoplasm, confluent large clusters situated in vacuoles with surrounding limiting membrane | black: 50–62 nm grey: 34–35 nm | Karnovsky’s fixative |
| 3 | Scattered throughout the anterior stroma, highest accumulation in the deep mid stroma | Intracellular, clusters in cytoplasm. Limiting membrane not preserved | black: 35–52 nm grey: 17–35 nm | retrieved from paraffin |
| 4 | Posterior third of stroma | Intracellular, diffusely in the entire cytoplasm. In between dense accumulations in clusters. Limiting membrane not preserved | approx 35 nm | retrieved from paraffin |
| 5 | Scattered throughout the entire stroma and within the fibrovascular subepithelial ingrowth | Intracellular, clusters in cytoplasm. Limiting membrane not preserved | black: 35–89 nm grey: 30–89 nm | band keratopathy, retrieved from paraffin |
| 6 | Paracentrally, just posterior to the epithelial basement membrane | Intracellular, clusters in cytoplasm. Limiting membrane not preserved | black: 61–39 nm grey: 22–61 nm | retrieved from paraffin |
| 7 | Scattered within individual fibroblasts in anterior and mid stroma | Intracellular, clusters in cytoplasm. Limiting membrane not preserved | black and grey: 28–45 nm | band keratopathy, retrieved from paraffin |