Table 2

Correlation of the results of conventional microbiological investigations and the PCR using universal eubacterial primers on 58 intraocular specimens from patients with clinical diagnosis of infective endophthalmitis and 20 intraocular specimens from non-infective disorders.

Type of endophthalmitis
(n=58)
(38 VF, 20 AH)
Infective endophthalmitis
Smear+, culture+
12 (12)
Smear+, culture− 4 (4)Smear−, culture+
4 (4)
Smear−, culture− 38 (17)
AH VF AH VF AH VF AH VF
Postoperative (n=36)4 (4)4 (4)2 (2)1 (1)2 (2)2 (2)8 (6)13 (6)
Post-traumatic (n=13)4 (4)1 (1)2 (0) 6 (4)
Endogenous (n=9)2 (0) 7(1)
  • AH=aqueous humour; VF=vitreous fluid.

  • += positive for bacteria; −= negative for bacteria.

  • Numbers in parentheses indicate PCR positivity.

  • Among 58 specimens, bacteriologically 20 (34.5%) were positive and 38 (65.5%) were negative.

  • 6 VF (three in the postoperative group and three in the endogenous group) and one AH (in the endogenous group) were positive for fungi but negative by PCR for eubacterial genome.

  • Among the 20 control specimens (six AH, 14 VF,) from non-infected cases, one was positive by PCR for eubacterial genome, and none by PCR for P acnes genome.