Detection of HSV-1, HSV-2, and VZV specific DNA fragments by PCR analysis on cornea derived DNA
| Diagnosis at PKP | No | HSV-1 | HSV-2 | VZV | ||||
|---|---|---|---|---|---|---|---|---|
| (positive results) | ||||||||
| I | HSK, primary graft | 20 | 8 | 0 | 5 | |||
| Allograft failure with initial HSK diagnosis | 11 | 2 | 1 | 1 | ||||
| II | Allograft failure with initial non-HSK diagnosis | 17 | 8 | 0 | 1 | |||
| Ulcers, non-specified | 9 | 3 | 0 | 1 | ||||
| III | Keratoconus | 15 | 0 | 0 | 2 | |||
| BKP | 14 | 2 | 0 | 2 | ||||
| Corneal dystrophies | 16 | 0 | 0 | 1 | ||||
| Corneal trauma, including chemical burns | 7 | 0 | 0 | 1 | ||||
| IV | Eye bank corneas | 23 | 1 | 0 | 0 | |||
Statistical analysis (χ2) showed significant differences in the amount of positive results (HSV-1 PCR) when group I was compared with IV, p=0.012 and group II was compared with III, p=0.003, and II compared with IV, p=0.003.
↵Three dual infections and two single VZV infections;
↵triple infection;
↵dual infections.