Tissue | Sex | Age | Pathology | Androgen receptor staining | |||||
---|---|---|---|---|---|---|---|---|---|
1st Antibody without competitor | 1st Antibody with “AR 462-478” peptide | 1st Antibody with “AR 1-21” peptide | |||||||
Lacrimal gland | female | 70 | + | + | − | ||||
Lacrimal gland | male | elderly | hypertrophy | + | + | − | |||
Meibomian gland | female | 48 | ectropion | + | + | − | |||
Meibomian gland | female | 70 | entropion | + | + | − | |||
Meibomian gland | male | 85 | + | + | − | ||||
Meibomian gland | male | 88 | chalazion | + | + | − | |||
Bulbar conjunctiva | female | 77 | + | + | − | ||||
Bulbar conjunctiva | female | 79 | cataract | + | + | − | |||
Bulbar conjunctiva | male | 27 | corneal scar and cataract | + | + | − | |||
Bulbar conjunctiva | male | 38 | keratoconus | + | + | − | |||
Bulbar conjunctiva | male | 70 | aphakia | + | + | − | |||
Bulbar conjunctiva | male | 85 | cataract | + | + | − | |||
Forniceal conjunctiva | male | 55 | + | + | − | ||||
Cornea | female | 65 | herpes simplex keratitis | + | − | ||||
Cornea | male | 27 | corneal scar and cataract | + | + | − | |||
Cornea | male | 38 | keratoconus | + | − | ||||
Lens epithelial cells | male | 13 | cataract | + | + | − | |||
RPE cells | male | 71 | cardiac arrest | + | + | − |
RPE = retinal pigment epithelial.
Tissues and cells were obtained and processed as described in the Materials and methods. To verify the specificity of staining, aliquots of the rabbit anti-human androgen receptor antibody were preincubated with excess amounts of either peptide “AR 1-21”, which was used to elicit the original antibody, or peptide “AR 462-478”, which was derived from a non-reactive part of the androgen receptor. These aliquots, or the first antibody alone (that is, without a competiting peptide), were then used in the immunoperoxidase procedure. Under these conditions, the AR 1-21, but not the AR 462-478, peptide should prevent specific androgen receptor staining.