Clinical details of each patient and the results of microbiologic culture and histological analysis of each biopsy specimen
| Patient No | Duration of symptoms before diagnosis (weeks) | Specimen No (type) | Antiamoebic therapy at the time of tissue biopsy | Duration of antiamoebic therapy at time of tissue biopsy (months) | Immunosuppression treatment at the time of tissue biopsy | Indication for tissue biopsy | Microbiology Culture | Histopathological analysis | Size of tissue used for histological analysis | |
|---|---|---|---|---|---|---|---|---|---|---|
| Cysts | Trophozoites | |||||||||
| 1 | 4 | 1 (PK) | PR+PHMB | 10 | TS, NSAID | Persistent Inflammation | Negative | Present | Not present | ½ corneal button |
| 2 | 16 | 2 (PK) | PR+PHMB | 21 | Nil | Scar excision | Positive | Present | Not present | ½ corneal button |
| 3 | 20 | 3 (PK) | PR+PHMB | 5 | TS | Persistent Inflammation | Negative | Present | Not present | ½ corneal button 8 mm |
| 4 (PK) | PHMB | 9 | TS, NSAID | Persistent Inflammation | Negative | Present | Present | ½ corneal button 9 mm | ||
| 5 (LCB) | PHMB | 12 | TS | Persistent Inflammation | Negative | Present | Present | 3×2×0.5mm | ||
| 6 (PK) | PHMB | 13 | TS | Persistent Inflammation | Negative | Present | Present | ½ corneal button | ||
| 4 | 10 | 7 (LCB) | PR | 1 | TS, NSAID | Excision of Necrotic tissue | Negative | Present | Not present | 4×2×1mm |
| 8 (PK) | PR | 4 | TS,SS, SC | Corneal perforation | Positive | Present | Present | ½ corneal button 7.5 mm | ||
| 5 | 17 | 9 (PK) | PR+PHMB | 16 | TS, SS | Persistent Inflammation | Positive | Not present | Not present | ½ corneal button |
| 6 | 4 | 10 (LCB) | PR+PHMB | 7 | TS | Persistent Inflammation | Positive | Not present | Not present | “Small fragment” |
| 11 (PK) | PR+PHMB | 8 | TS | Descemetocoele | Negative | Present | Not present | ½ corneal button | ||
| 7 | 24 | 12 (LCB) | PR+PHMB | 11 | Nil | Scar excision | Negative | Present | Not present | ½ corneal button 0.25 mm |
| 8 | 16 | 13 (LCB) | PHMB | 7 | TS | Persistent Inflammation | Negative | Not present | Not present | “Small fragment” |
| 14 (PK) | PR+PHMB | 16 | TS, NSAID | Corneal perforation | Positive | Present | Not present | ½ corneal button 4 mm | ||
| 9 | 0.5 | 15 (LCB) | CH+PHMB | 6 | TS, NSAID | Persistent Inflammation | Negative | Not present | Not present | 1.5×1×0.5mm |
| 10 | 16 | 16 (LCB) | CH | 2 | Nil | Persistent Inflammation | Negative | Present | Not present | 2 of 1×1×0.5mm |
| 17 (PK) | CH+HX | 4 | TS, SS, SC | Corneal perforation | Positive | Present | Not present | ½ corneal button6×7 mm | ||
| 11 | 6 | 18 (PK) | PR | 4 | TS,NSAID, SS | Corneal perforation | Negative | Present | Not present | ¼ corneal button 6×7 mm |
| 12 | 3 | 19 (LCB) | PR | 10 | TS | Persistent Inflammation | Negative | Present | Present | Not recorded |
| 20 (LCB) | PHMB | 21 | TS | Persistent Inflammation | Negative | Present | Not present | Not recorded | ||
| 21 (PK) | PHMB | 31 | TS | Scar excision | Negative | Present | Not present | ½ corneal button 7 mm | ||
| 13 | 2 | 22 (LCB) | PR+PHMB | 2 | TS, NSAID | Persistent Inflammation | Negative | Present | Not present | 2mm fragment |
| 23 (LCB) | PR+PHMB | 3 | TS, NSAID | Persistent Inflammation | Negative | Present | Not present | 1mm fragment | ||
| 14 | 4 | 24 (LCB) | PR+PHMB | 21 | TS | Scar excision | Negative | Present | Not present | Not recorded |
↵Specimens obtained from patients with no evidence of clinical inflammation at the time of tissue biopsy.
PK = penetrating keratoplasty; LCB = lamellar corneal biopsy; PR = propamidine isethionate; PHMB = polyhexamethyl biguanide; CH = chlorhexidine; HX = hexamidine; TS = topical steroids; NSAID = systemic non-steroidal anti-inflammatory drugs; SS = systemic steroids (prednisolone); SC = systemic cyclosporine.