Amplicon | Primer name | Sequence | Size (bp) | Restriction enzyme (sizes in bp) | TA (°C) |
1 | 91.R7 | 5′-TAATCCCTTTCTGTTTCTGG-3′ | 348 | NlaIII (201+147) | 53 |
91.S4 | 5′-AGGACAAGAAGACAATGAAG-3′ | ||||
2 | 91.R1 | 5′-ATCTCATCCACACAGCCAC-3′ | 318 | Mnl I (180+138) | 59 |
91.S8 | 5′-CGATCACTGCCACTGTCTTC-3′ | ||||
3 | 91.R2 | 5′-CCAGTTCAGGCTTCATCATC-3′ | 439 | Acc I (257+182) | 60 |
91.S7 | 5′-CCGGCATGGCACACTGGAT-3′ | ||||
4 | 91.R3 | 5′-TGTGCAGGGAGGTGGAGAT-3′ | 303 | Mnl I (172+131) | 60 |
91.S6 | 5′-AAACTGGTAAAGAATGAAGC-3′ | ||||
5 | 91.R4 | 5′-GTCCTGGTGTGCTGTCTTCC-3′ | 327 | Hae III (196+131) | 56 |
91.S2 | 5′-CTGTGGCTTTGGAGATAACA-3′ | ||||
6 | 91.R5 | 5′-CAGAAACAAATCCTCCCATC-3′ | 349 | Alu I (190+159) | 60 |
91.S1 | 5′-TCTGCTTGGCTGAAGTGCTG-3′ | ||||
7 | 91.R9 | 5′-AACTCTTTTGGATTTGCC-3′ | 248 | - | 54 |
91.S11 | 5′-GAAAGAAAACCATAACTGCC-3′ |
↵PCR conditions were 94°C, 5 minutes; 30 cycles of 94°C, 30 seconds, TA, 30 seconds, 72°C, 30 seconds; 72°C, 5 minutes.