Endothelial cell numerical densities and losses incurred (Δ) by 17 (I–XVII) fellow pairs (right eye and left eye) of donor corneas after an initial 10 day phase and after medium change by 12 fellow pairs (I–XII) after another 10 day phase
| Corneal pair no | Cell density/mm2 | Total cell loss | |||
|---|---|---|---|---|---|
| Before organ culture | After 10 days of organ culture | After 20 days of organ culture | |||
| I RE | 2500 | 2400 (Δ 100) | 2100 (Δ 300) | 400 | |
| I LE | 2500 | 2300 (Δ 200) | 2250 (Δ 50) | 250 | |
| II RE | 2150 | 2200 (Δ +50) | 1750 (Δ 450) | 400 | |
| II LE | 2150 | 2200 (Δ +50) | 2100 (Δ 50) | 50 | |
| III RE | 2400 | 2200 (Δ 200) | 2300 (Δ+100) | 100 | |
| III LE | 2400 | 2200 (Δ 200) | 2300 (Δ+100) | 100 | |
| IV RE | 2500 | 2200 (Δ 300) | 2150 (Δ 50) | 350 | |
| IV LE | 2500 | 2200 (Δ 300) | 2200 (Δ 0) | 300 | |
| V RE | 2500 | 2200 (Δ 300) | 2100 (Δ 100) | 400 | |
| V LE | 2500 | 2200 (Δ 300) | 2100 (Δ 100) | 400 | |
| VI RE | 2400 | 2400 (Δ 0 ) | 2300 (Δ 100) | 100 | |
| VI LE | 2400 | 2400 (Δ 0 ) | 2300 (Δ 100) | 100 | |
| VII RE | 2300 | 2200 (Δ 100) | 1950 (Δ 250) | 350 | |
| VII LE | 2100 | 2100 (Δ 0) | 2050 (Δ 50) | 50 | |
| VIII RE | 2500 | 2500 (Δ 0) | 2300 (Δ 200) | 200 | |
| VIII LE | 2600 | 2500 (Δ 100) | 2350 (Δ 150) | 250 | |
| IX RE | 2200 | 2150 (Δ 50) | 1500 (Δ 650) | 700 | |
| IX LE | 2200 | 2150 (Δ 50) | 2050 (Δ 100) | 150 | |
| X RE | 2700 | 2700 (Δ 0) | 2500 (Δ 200) | 200 | |
| X LE | 2700 | 2700 (Δ 0) | 2500 (Δ 200) | 200 | |
| XI RE | 2500 | 2450 (Δ 50) | 2200 (Δ 250) | 300 | |
| XI LE | 2350 | 2450 (Δ+100) | 2250 (Δ 200) | 100 | |
| XII RE | 2250 | 2150 (Δ 100) | 2100 (Δ 50) | 150 | |
| XII LE | 2250 | 2150 (Δ 100) | 2100 (Δ 50) | 150 | |
| XIII RE | 2200 | 2050 (Δ 50) | |||
| XIII LE | 2200 | 2200 (Δ 0) | |||
| XIV RE | 2100 | 2000 (Δ 100) | |||
| XIV LE | 2250 | 2150 (Δ 100) | |||
| XV RE | 2200 | 2200 (Δ 0) | |||
| XV LE | 2200 | 2150 (Δ 50) | |||
| XVI RE | 2100 | 2050 (Δ 50) | |||
| XVI LE | 2100 | 1950 (Δ 150) | |||
| XVII RE | 2400 | 2400 (Δ 0) | |||
| XVII LE | 2400 | 2400 (Δ 0) | |||
The left corneal cultures (LE) were supplemented with 25 μg/ml pentoxifylline, right corneal cultures (RE) were cultured under standard conditions and served as controls.