Table 1

Specifications of the eight test media

Name	Basic component	% Serum supplementation	Comments
MEM-8%	MEM-Earle (Seromed)	8	Commonly used for organ culture in European eye banks¹
MEM-2%	MEM-Earle (Seromed)	2	Commonly used for organ culture in European eye banks¹
MEM-G	MEM-Earle (Seromed)	2	MEM supplemented with 2 mM l-analyl-l-glutamine, a stabilised form (dipeptide) of the amino acid l-glutamine
F99	M199 + Ham's F12 (1:1) (Gibco)	2	Used for corneal endothelial cell growth14
F99-Sr	M199 + Ham's F12 (1:1) (Gibco)	2	F99 supplemented with ascorbic acid, insulin, bFGF, transferrin, selenium, and lipids14
M199	M199-Earle (Seromed)	2	Intended for short term corneal preservation at 4°C (cold storage for up to 10 days)
SFM	Company formula (Gibco)	2	Developed for serum free growth of vascular endothelial cells
DIF-1000	Company formula (Seromed)	2	Initially used for serum free growth of hybridoma cells

See company information sheets for further details on the specific composition of each medium.