Antibodies used for immunohistochemistry: all were polyclonal affinity purified
| Antibody | Supplier | Catalogue number | Antibody | Dilution used | Pretreatment |
|---|---|---|---|---|---|
| Calbiochem/Oncogene Research Products, Nottingham, UK. | |||||
| R&D Systems UK Ltd, Santa Cruz, Autogen Bioclear UK Ltd, Calne, Wilts, UK. | |||||
| Dako Ltd, Ely, UK. | |||||
| vWF | Dako | M616 | Mouse IgG | 1:500 | Trypsin 30 minutes |
| bFGF | Calbiochem | PC194L | Goat IgG | 1:20 | Trypsin 15 minutes |
| VEGF (1) | Calbiochem | PC315 | Rabbit IgG | 1:20 | Trypsin 15 minutes |
| VEGF (2) | R&D Systems | AB-293-NA | Goat IgG | 1:200 | Trypsin 15 minutes |
| VEGF (3) | Santa Cruz | SC-152 | Rabbit IgG | 1:75 | Pressure cook in citrate buffer pH 6.0 for 3.5 minutes on high and 10 minutes on medium power, then 0.015% trypsin at 37°C for 45 minutes |