N = 30* | Culture positive for fungi | Culture negative for fungi |
---|---|---|
*Percentages of the total of 30 specimens are shown in parentheses | ||
†Specimens were considered to be “PCR positive” if a positive DNA product was obtained using any 1 of the 3 primer sets described. | ||
‡These fungal culture isolates were examined microscopically and recorded as “hyaline” or “dematiaceous.” They were not speciated. | ||
PCR positive for fungi† | 15 (50%) | 7 (23%) |
Culture results: | Clinical impression: | |
Fusarium: 5 (17%) | Fungal keratitis: 2 (7%) | |
Aspergillus 2 (7%) | Bacterial keratitis: 4 (13%) | |
Unidentified: 8 (27%)‡ | Uncertain: 1 (3%) | |
PCR negative for fungi | 1 (3%) | 7 (23%) |
Culture result: unidentified‡ | Culture results: | |
Bacteria: 4 (13%) | ||
Clinical impression: fungal keratitis | Acanthamoeba: 2 (7%) | |
No growth: 1 (3%) | ||
16 (54%) | 14 (46%) |