Table 2

Real time RT-PCR primers and conditions

Gene name	GenBankAcc No		Sequence	Location	Size (BP)	Cycles	Tm (°C)
SEC13-like 1 (S cerevisiae)	AA496784	F	CGTGTGTTCATTTGGACCTG	868–1103	236	45	55
SEC13-like 1 (S cerevisiae)	AA496784	R	CCCTCTGTCACTGATGCTGA	868–1103	236	45	55
Topoisomerase (DNA) II α170 kDa	AA504348	F	TCCTGCCAAAACCAAGAATC	4494–4666	173	45	55
Topoisomerase (DNA) II α170 kDa	AA504348	R	GTACAGATTTTGCCCGAGGA	4494–4666	173	45	55
ATPase, H+ transporting, lysosomal V0 subunit a isoform 1	AA427472	F	TGCCCTGCACTACATAGCAC	3326–3514	189	45	55
ATPase, H+ transporting, lysosomal V0 subunit a isoform 1	AA427472	R	GGGGAAGATCTCAGGGTCTC	3326–3514	189	45	55
Oxidase (cytochrome c) assembly 1-like	AA598582	F	GTCGAATCAGAGAGGCCAAG	722–948	227	45	55
Oxidase (cytochrome c) assembly 1-like	AA598582	R	GAGATCCTGGAACCACCAGA	722–948	227	45	55
START domain containing 4, sterol regulated	H11369	F	ACCGCTCAAGGGGTTATTCT	979–1158	180	45	55
START domain containing 4, sterol regulated	H11369	R	CCAACACTTTGGGAGGCTAA	979–1158	180	45	55
Acidic ribosomal phosphoprotein PO	M17885	F	CGACCTGGAAGTCCAACTAC	93–201	109	45	53
Acidic ribosomal phosphoprotein PO	M17885	R	ATCTGCTGCATCTGCTTG	93–201	109	45	53