Detection of Sry (Y chromosome) gene product by PCR in solid organs harvested from two bone marrow chimeric (Y→X) Lewis rats for each of five time points after bone marrow transplant
| 8 weeks | 12 weeks | 15 weeks | 30 weeks | 52 weeks | |
|---|---|---|---|---|---|
| Detection of gene product by PCR is denoted with a plus or minus for each animal. Thus, “+/−” denotes detection of Sry in the first animal, but not the second. There was a rapid repopulation of the spleen (at 8 weeks) and, to a lesser extent, lung (at 30 weeks) in Y→X bone marrow transplantation. This repopulation was absent in the brain parenchyma and retina until 52 weeks after transplantation. | |||||
| Spleen | +/+ | +/+ | +/+ | +/+ | +/+ |
| Lung | −/− | −/− | −/− | +/+ | +/+ |
| Right retina | −/− | −/− | −/− | −/− | −/− |
| Left retina | −/− | −/− | −/− | −/− | +/+ |
| Brain | −/− | −/− | −/− | −/− | +/+ |