Percentage of TUNEL positive nuclei in grafted rat corneas at various times after transplantation
| Specimen | No of eyes* | Mean (SD) % TUNEL + nuclei in corneal stroma | |
|---|---|---|---|
| Without dUTP† | With dUTP‡ | ||
| *Only one eye of any rat was grafted for ethical reasons, therefore number of eyes represents number of rats. | |||
| †Control for TUNEL reaction (biotinylated dUTP omitted from reaction). | |||
| ‡Test for TUNEL reaction (biotinylated dUTP included in reaction). | |||
| Normal corneas | 5 | 1.7 (2.3) | 29.3 (15.3) |
| Isograft day 0 | 3 | 1.5 (3.1) | 35.3 (17.6) |
| Isograft day 5 | 3 | 1.7 (2.7) | 50.1 (19.2) |
| Isograft day 10 | 3 | 0.3 (0.8) | 33.9 (14.8) |
| Isograft day 14 | 3 | 1.5 (1.4) | 47.9 (28.8) |
| Isograft day 21 | 3 | 0.4 (0.9) | 65.0 (22.8) |
| Isograft day >60 | 4 | 0.9 (0.9) | 42.5 (16.3) |
| Allograft day 0 | 3 | 2.1 (4.0) | 29.2 (19.9) |
| Allograft day 5 | 3 | 2.4 (3.0) | 57.8 (10.4) |
| Allograft day 10 | 3 | 0.8 (0.9) | 75.3 (12.4) |
| Allograft day 15 | 3 | 8.4 (4.7) | 93.6 ( 6.3) |
| Allograft day 21 | 3 | 0.8 (1.4) | 78.3 (18.0) |