Table 2 Amplicon location, number of CpG sites, primer sequences and PCR conditions

Gene	GenBankaccession No	Amplicon locationrelative totranscriptionstart site	CpGsites	PCR conditions
Gene	GenBankaccession No	Amplicon locationrelative totranscriptionstart site	CpGsites	PCR	Primer sequences (5′–3′)	Tm(°C)	MgCl₂(mM)	DMSO
p16	AF0022809	−46 to +126		Outer	GGGGGAGATTTAATTTGGCAAC CCCTCCTCTTTCTT	54	1.5	5%
			10	Inner	GATTTTAGGGGTGTTATATTCAA CCCCTCCTCTTTCTT	54	1.5	–
TIMP3	AF001361	−189 to −36		Outer	GTATTATTTTTTATAAGGATTTG CCTACCTACTACCCTCTCTAC	50	2.0	–
			11	Inner	GGTTTAGTTTTTTTTTTGGAGCC TACCTACTACCCTCTCTAC	50	2.0	–
RASSF1	AC002481	−2 to +167		Outer	CCCCACAATCCCTACACCCAAA TGTTTTGGTAGTTTAATGAGTTT AGGTTTTTT	55	1.5	–
			14	Inner	ACTCTCCTCAACTCCTTCCGTTT TGGTAGTTTAATGAGTTTAGGTT TTTT	54	1.5	–
RARB	X56849	−306 to −150		Outer	GTAAAGGGAGAGAAGTTGGTGT CAACTCCCAAAATTCTCACAA	55	1.5	5%
			7	Inner	AAGGGAGAGAAGTTGGTGTTTA AAACAAACAACCAAAAAAACA AA	50	1.5	5%
FHIT	AF399855/NM_002012	−126 to +42		Outer	GTTTTTGAATTAGGGTTATTGTT CCTAAAAATATACCCACTAAAT A	53	2.0	–
			11	Inner	GTTATTATGGTTTTTAATTGGTT CAAAAAACAAAAAATCCTATAA C	50	2.0	–
hTERT	AF128893	−387 to −219		Outer	GGGTTATTTTATAGTTTAGGTAA TCCCCAATCCCT	53	2.0	5%
			20	Inner	GGGTTATTTTATAGTTTAGGTAA TCCACTAAAAACCC	48	1.5	5%
APC	U02509	−210 to −52		Outer	GGGGTTAGGGTTAGGTAGGAAC TACACCAATACAACCACATA	53	1.5	5%
			13	Inner	GGGTTAGGGTTAGGTAGGTTGT CCCACACCCAACCAATC	53	1.5	5%