| Herpes virus | Sequence for primers and probes | Amplification |
| HSV1 and 2 | HSV-F: CGCATCAAGACCACCTCCTC | gB |
| HSV-R: GCTCGCACCACGCGA | ||
| HSV1-P: JOE-TGGCAACGCGGCCCAAC-TAMRA | ||
| HSV2-P: FAM-CGGCGATGCGCCCCAG-TAMRA | ||
| VZV | VZV-F: AACTTTTACATCCAGCCTGGCG | ORF29 |
| VZV-R: GAAAACCCAAACCGTTCTCGAG | ||
| VZV-P: FAM-TGTCTTTCACGGAGGCAAACACGT-TAMRA | ||
| EBV | EBV-F: CGGAAGCCCTCTGGACTTC | BALF5 |
| EBV-R: CCCTGTTTATCCGATGGAATG | ||
| EBV-P: FAM-TGTACACGCACGAGAAATGCGCC-TAMRA | ||
| CMV | CMV-F: CATGAAGGTCTTTGCCCAGTAC | IE-1 |
| CMV-R: GGCCAAAGTGTAGGCTACAATAG | ||
| CMV-P: FAM-TGGCCCGTAGGTCATCCACACTAGG-TAMRA | ||
| HHV6 | HHV6-F: GACAATCACATGCCTGGATAATG | U65-U66 |
| HHV6-R: TGTAAGCGTGTGGTAATGTACTAA | ||
| HHV6-P: FAM-AGCAGCTGGCGAAAAGTGCTGTGC-TAMRA | ||
| HHV7 | HHV7-F: CGGAAGTCACTGGAGTAATGACAA | U37 |
| HHV7-R: CCAATCCTTCCGAAACCGAT | ||
| HHV7-P: FAM-CTCGCAGATTGCTTGTTGGCCATG-TAMRA | ||
| HHV8 | HHV8-F: CCTCTGGTCCCCATTCATTG | ORF65 |
| HHV8-R: CGTTTCCGTCGTGGATGAG | ||
| HHV8-P: FAM-CCGGCGTCAGACATTCTCACAACC-TAMRA |
The real-time herpes simplex virus (HSV) PCR is a multiplexing PCR that can detect both HSV1 and HSV2 DNA in the same reaction. The optimised gB primer pairs amplify both HSV1 and 2 with equal efficiency, with the two type-specific probes labelled with different fluorescent dyes. HSV1 probe is labelled with JOE at the 5′-end and with TAMRA at the 3′-end. HSV2 probe is labelled with FAM at the 5′-end and with TAMRA at the 3′-end.
CMV, cytomegalovirus; EBV, Epstein–Barr virus; VZV, Varicella-zoster virus.