Primers used in Sanger sequencing
| Primer name | Forward primer (5′–3′) | Reverse primer (5′–3′) | Amplicon (bp) | Annealing temperature (°C) | |
|---|---|---|---|---|---|
| KIF11-E2-94366076 | tccatcaccaacaatgtggtc | tcagcccactagaagccatc | 387 | 65–58 | |
| KIF11-E17-94399620 | tcacttccactgctttcctct | agagagcacaaatacgcaatga | 469 | 65–58 | |
| KIF11-E20-94409684 | acaggtagcaagactgatcct | cggggtaagattgaggggta | 400 | 65–58 | |
| KIF11-E21-94410186 | caatcttaccccgcctctca | gttgttctgctcaccacgaa | 531 | 65–58 |