Regular Article
Protein Tyrosine Kinases Expressed in Glomeruli and Cultured Glomerular Cells: Flt-1 and VEGF Expression in Renal Mesangial Cells

https://doi.org/10.1006/bbrc.1995.1492Get rights and content

Abstract

Protein tyrosine kinases play an important role in cellular proliferation and differentiation of various cell types. To identify potential tyrosine kinases involved in glomerular functions we have utilized the polymerase chain reaction (PCR) and degenerate oligonucleotides for isolation of such genes from isolated glomeruli, cultured mesangial cell, and glomerular endothelial cells. Sequence analysis of PCR-amplified cDNAs resulted in the isolation of 24 tyrosine kinases. Here we describe for the first time the constitutive expression of 15 tyrosine kinases, tyro-1, tyro-4, tyro-6, hyk, Ptk-3, Ryk, tie, yes, lyn, tec, Jak1, Jak2, Jak3, c-abl, and flk, in renal glomeruli. In addition, Flt-1, an endothelial cell-specific receptor for vascular endothelial growth factor (VEGF), is expressed in renal mesangial cells and its gene expression is up-regulated upon the stimulation of platelet-derived growth factor (PDGF) with the concomitant up-regulation of VEGF. These data suggest the possible involvement of VEGF/Flt-1 system in cytokine-induced mesangial cell proliferations.

References (0)

Cited by (81)

  • Hypoxia inhibits nephrogenesis through paracrine Vegfa despite the ability to enhance tubulogenesis

    2015, Kidney International
    Citation Excerpt :

    Moreover, using two different antibodies directed against Vegfa, branching could be rescued considerably in the presence of ‘hypoxic medium’ (Figure 5c). Since there are contradictory reports regarding the expression of Vegf receptors in MDCK cells,36,37 we tested for the expression of Vegf receptor 1 (Flt-1) and Vegf receptor 2 (Flk-1) in the MDCK cells used in our experiments. We found that Flt-1, but not Flk-1 is expressed in these cells (Supplementary Figure S4A online).

  • Vasopressin induces human mesangial cell growth via induction of vascular endothelial growth factor secretion

    2011, Neuropeptides
    Citation Excerpt :

    However, the precise role of VEGF in the kidney is unknown. While renal biopsy studies have indicated that VEGF receptors are predominantly expressed on glomerular endothelial cells, faint expression has also been detected in the mesangial area, and expression has also been demonstrated in cultured rat and human mesangial cells (Takahashi et al., 1995; Thomas et al., 2000). In addition, human and rat mesangial cells have been proven to produce VEGF, synthesis of which is stimulated by cytokines that are important regulators of mesangial cell functions during development of glomerulopathies such as transforming growth factor-β (TGF-β) and platelet-derived growth factor (PDGF) (Takahashi et al., 1995; Iijima et al., 1993; Finkenzeller et al., 1992).

  • Vascular endothelial growth factor isoforms and their receptors are expressed in human osteoarthritic cartilage

    2003, American Journal of Pathology
    Citation Excerpt :

    In our study, all of the OA cartilage samples expressing VEGFR-2 also expressed NRP-1. VEGFR-1 and VEGFR-2 were originally thought to be expressed predominantly in endothelial cells, but recent studies have indicated that other types of cells also express one or both of the receptors: VEGFR-1 is expressed in trophoblasts,31 monocytes,32 and mesangial cells,33 and VEGFR-2 is expressed in hematopoietic stem cells, megakaryocytes,34 and retinal progenitor cells.35 The present data add OA chondrocytes in the list, and suggest the possible direct actions of VEGF on the articular chondrocytes in OA.

View all citing articles on Scopus
View full text