Gender and Androgen Treatment Influence the Expression of Proto-oncogenes and Apoptotic Factors in Lacrimal and Salivary Tissues of MRL/lprMice

doi:10.1006/clin.1997.4466

Clinical Immunology and Immunopathology

Volume 86, Issue 1, January 1998, Pages 59-71

Clinical Immunology ...

https://doi.org/10.1006/clin.1997.4466 Get rights and content

Abstract

The objectives of this study were to (1) determine whether Fas antigen, Fas ligand, p53, and proto-oncogene mRNAs may be detected in lacrimal and submandibular glands of the MRL/lprmouse model of Sjögren's syndrome, and (2) examine whether gender and androgen or cyclophosphamide therapy influence the mRNA expression of these apoptotic factors. Tissues were obtained from treated or untreated MRL/lprmice after the onset of disease and processed for the analysis of mRNAs by RT-PCR and Southern blot hybridization. Our results demonstrated that (1) Fas antigen (exons 1 → 2 or 3 → 7+), Fas ligand, c-myb, c-myc, bcl-2, Bax, p53, and androgen receptor (AR) mRNAs are present in exocrine tissues of MRL/lprmice; (2) the amounts of c-myb, c-myc, bcl-2, p53, and AR mRNA are higher (P< 0.05) and the level of Fas antigen (exons 1 → 2) mRNA is lower (P< 0.05) in lacrimal glands of female compared to male mice. In contrast, the content of c-myb and p53 mRNA is greater (P< 0.05) in submandibular tissues of female relative to those of male mice; and (3) testosterone or cyclophosphamide treatment led to a significant (P< 0.05) decline in the mRNA levels of c-myb, bcl-2, and/or AR, but an increase (P< 0.05) in the mRNA amount of Bax, in lacrimal, but not in salivary, glands of female mice. These findings demonstrate that gender-associated differences exist in the expression of apoptotic factor mRNAs in exocrine tissues of autoimmune mice and that some of these differences appear to be due to the influence of androgens.

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Regular ArticleGender and Androgen Treatment Influence the Expression of Proto-oncogenes and Apoptotic Factors in Lacrimal and Salivary Tissues of MRL/lprMice☆

Abstract

Bailliere's Clin. Rheum.

J. Ster. Biochem. Mol. Biol.

J. Autoimmun.

Clin. Immunol. Immunopathol.

Clin. Immunol. Immunopathol.

Cell

Clin. Immunol. Immunopathol.

J. Autoimmunity

Clin. Immunol. Immunopathol.

Cell

Anal. Biochem.

J. Ster. Biochem. Mol. Biol.

Clin. Immunol. Immunopath.

Clin. Immunol. Immunopathol.

J. Steroid Biochem. Mol. Biol.

Exp. Eye Res.

Sjögren's Syndrome. State of the Art,

Possible mechanisms involved in the reduced tear secretion in Sjögren's syndrome

Sjögren's Syndrome. State of the Art

Sjögren's syndrome: Immunologic and neuroendocrine mechanisms

Adv. Exp. Med. Biol.

Clinical diagnosis of the dry eye

Int. Ophthalmol. Clin.

Keratoconjunctivitis sicca

Importance of sex hormones in systemic lupus erythematosus

Oestrogen is a potent disease accelerator in SLE-prone MRLlpr/lpr

Clin. Exp. Immunol.

Lymphoproliferation disorder in mice explained by defects in Fas antigen that mediates apoptosis

Nature

The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen

J. Immunol.

Aberrant transcription caused by the insertion of an early transposable element in an intron of the Fas antigen gene oflpr

Proc. Natl. Acad. Sci. USA

The defect in Fas mRNA expression in MRL/lpr

J. Exp. Med.

The Fas protein is expressed at high levels on CD4++lpr/lpr

Proc. Natl. Acad. Sci. USA

Mature CD4+

J. Immunol.

Correction of accelerated autimmune disease by early replacement of the mutatedlprlpr/lpr

Proc. Acad. Natl. Sci. USA

Autoimunity and increased c-myb transcription

Science

Studies of c-myb gene regulation in MRL-lpr/lprlpr-lpr

J. Immunol.

c-myc mRNA expression in minor salivary glands of patients with Sjögren's Syndrome

J. Rheumatol.

Constitutive activation of the Fas ligand gene in mouse lymphoproliferative disorders

EMBO J.

Massive upregulation of the Fas ligand inlprgld

J. Exp. Med.

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J. Exp. Med.

Oncogenes, growth factors and autoimmune diseases

Anticancer Res.

Increased proto-oncogene expression in peripheral blood T lymphocytes from patients with systemic sclerosis

Arthritis Rheum.

Regular Article
Gender and Androgen Treatment Influence the Expression of Proto-oncogenes and Apoptotic Factors in Lacrimal and Salivary Tissues of MRL/lprMice☆

The Fas protein is expressed at high levels on CD4⁺⁺lpr/lpr

Mature CD4⁺