Skip to main content
Log in

Automated video image morphometry of the corneal endothelium

  • Published:
Documenta Ophthalmologica Aims and scope Submit manuscript

Abstract

The central corneal endothelium of 13 eyes in 13 subjects was visualized with a non-contact specular microscope. This report describes the computer-assisted morphometric analysis of enhanced digitized images, using a direct input by means of a frame grabber. The output consisted of mean cell area, cell density, frequency distribution of the individual cell area, and cell polygonality. Results showed that the mean coefficient of variation of images analyzed three times consecutively was 0.95 percent. The cell analysis of three different images of the same subject was accurate, with a mean coefficient of variation of 4.2 percent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Maurice DM. Cellular membrane activity in the corneal endothelium of the intact eye. Experientia 1968; 24: 1094–95.

    Google Scholar 

  2. Laing RA, Sandstrom MM, Leibowitz HM, In vivo photomicrography of the corneal endothelium. Arch Ophthalmol 1977; 93: 143–45.

    Google Scholar 

  3. Olsen T. Non-contact specular microscopy of human corneal endothelium. Acta Ophthalmol 1979; 57: 986–98.

    Google Scholar 

  4. Bourne WM, Kaufman H. Specular microscopy of human corneal endothelium in vivo. Am J Ophthalmol 1976; 81: 319–23.

    Google Scholar 

  5. Sherrard ES, Kerr MG. Routine specular microscopy: Expectations and limitations. Seminars in Ophthalmology 1986: 221–31.

  6. Sugar A. Clinical specular microscopy. Surv Ophthalmol 1979; 24: 21–32.

    Google Scholar 

  7. Price MN, Cheng H. Contact and noncontact specular microscopy. Br J Ophthalmol 1981; 65: 568–74.

    Google Scholar 

  8. Ullern M. La microscopie spéculaire. J Fr Ophthalmol 1988; 11: 525–40.

    Google Scholar 

  9. American Academy of Ophthalmology. Corneal endothelial photography. Ophthalmology 1991; 98: 1464–68.

    Google Scholar 

  10. Waring GO, Bourne WM, Edelhauser HF, Kenyon KR. The corneal endothelium: Normal and pathologic structure and function. Ophthalmology 1982; 89: 531–90.

    Google Scholar 

  11. Waring GO, Krohn MA, Ford GE, Harris RR, Rosenblatt LS. Four methods of measuring human corneal endothelial cells from specular micrography. Arch Ophthalmol 1980; 98: 848–55.

    Google Scholar 

  12. Lester JM. McFarland JL, Bursell SE, Laing RA, Brenner JF. Automated morphometric analysis of corneal endothelial cells. Invest Ophthalmol Vis sci 1981; 20: 407–10.

    Google Scholar 

  13. Fabian E, Mertz M, Köditz W. Endothelmorphometrie durch automatisierte Fernsehbildanalyse. Klin Mbl Augenheilk. 1983; 182: 218–23.

    Google Scholar 

  14. Hirst LW, Sterner RE, Grant DG. Automated analysis of wide-field specular photomicrographs. Cornea 1984; 3: 83–87.

    Google Scholar 

  15. Rao GN. Morphometry of corneal endothlium: Editorial. Cornea 1984/1985; 3: 153–54.

    Google Scholar 

  16. Hartmann C, Köditz W. Automated morphometric endothelial analysis combined with video specular microscopy. Cornea 1984/1985; 3: 155–67.

    Google Scholar 

  17. Laing RA. Image processing of corneal endothelial images. In: Cavanagh HD, ed. The cornea: Transactions of the world congress on the cornea, III 1988: 259–65.

  18. Nishi O, Hanasaki K. Automated morphometry of corneal endothelial cell: Use of video camera and video tape recorder. Br J Ophthalmol 1988; 72: 68–73.

    Google Scholar 

  19. Nishi O, Hanasaki K. Automated determination of polygonality of corneal endothelial cells. Cornea 1989; 8: 54–57.

    Google Scholar 

  20. Koester CJ, Roberts CW, Donn A, Hoefle FB. Wide field specular microscopy: Clinical and research applications. Ophthalmology 1980; 87: 849–90.

    Google Scholar 

  21. Laing RA, Sandstrom MM, Berrospi AR, et al. Changes in the corneal endothelium as a function of age. Exp Eye Res 1976; 22: 587–94.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Siertsema, J.V., Landesz, M., Van Den Brom, H. et al. Automated video image morphometry of the corneal endothelium. Doc Ophthalmol 85, 35–44 (1993). https://doi.org/10.1007/BF01268098

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01268098

Key words

Navigation