Abstract
The corneal endothelium of 179 healthy persons (327 eyes), 4–89 years of age, was photographed through a non-contact specular microscope. From the histograms of the cell areas of each eye, other parameters were derived and subjected to computerized statistical analysis. In addition, photographs of both eyes of one person were taken on two different days and examined to determine the reproducibility of the method.
The known age-dependent increase in the mean cell area as well as the decrease of the cell density with age could be confirmed. Other parameters that increase with age are the variability of the mean cell area and the maximum cell area; the latter correlates so well with age and is so easily measured that this parameter may facilitate further morphometric evaluations. For these age-dependent correlations not only regression lines but also third-rank regression curves, which seem to fit better, were calculated. Finally,age-independent parameters were found that may serve as potential additional criteria for a qualitative evaluation of the individual corneal endothelium.
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References
Bigar F, Witmer R, Thaer A (1978) Die Spiegelmikroskopie des Hornhautendothels (Specular microscopy). Klin Monatsbl Augenheilkd 173: 691–696
Blatt HL, Rao GN, Aquavella JV (1979) Endothelial Cell Density in Relation to Morphology. Invest Ophthalmol Vis Sci 18: 856–860
Bourne WM, Kaufman HE (1976) Specular Microscopy of Human Corneal Endothelium in Vivo. Am J Ophthalmol 81: 319–323
Hirst S et al. (1980) Clinical specular microscopy (Editorial). Invest Ophthalmol Vis Sci 19: 2–4
Irvine AR, Irvine, Jr. AR (1953) Variations in Normal Human Corneal Endothelium. Am J Ophthalmol 36: 1279–1285
Kaufman HE, Capella JA, Robbins JE (1966) The Human Corneal Endothelium. Am J Ophthalmol 61: 835–841
Kontron Firmenschrift MOP/AMOZ AMO3: Das Baukastensystem zur halbautomatischen Erfassung bildanalytischer Daten. Fa Kontron, Oscar-v-Miller-Str 1, D-8057 Eching
Laing RA et al. (1976) Changes in the Corneal Endothelium as a Function of Age. Exp Eye Res 22: 587–594
Laing RA, Sandstrom MM, Leibowitz HM (1979) Clinical Specular Microscopy. II. Qualitative Evaluation of Corneal Endothelial Photomicrographs. Arch Ophthalmol (Chicago) 97: 1714–1719
Laule A et al. (1978) Endothelial Cell Population Changes of Human Cornea During Life. Arch Ophthalmol (Chicago) 96: 2031–2035
Olsen T (1979) Optical Principles for Estimation of Endothelial Cell Density with the Non-Contact Specular Microscope. Acta Ophthalmol (Kbh) 57: 860–867
Olsen T (1979) Non-Contact Specular Microscopy of Human Corneal Endothelium. Acta Ophthalmol (Kbh) 57: 986–998
Stanley et al. (1980) A study in the measurement of corneal endothelial cell density using the specular microscope. Acta Ophthalmol (Kbh) 58: 418–423
Sturrock GD, Sherrard ES, Rice NSC (1978) Specular Microscopy of the Corneal Endothelium. Br J Ophthalmol 62: 809–814
Sundmacher R, Müller O (1980) Spaltlampenmikroskopie des Hornhautendothels im Spiegelbezirk. Ber Zusammenkunft Dtsch Ophthalmol Ges 77: 943–950
Vogt A (1919) Die Sichtbarkeit des lebenden Hornhautendothels im Lichtbüschel der Gullstrandschen Spaltlampe. Klin Monatsbl Augenheilkd 63: 233–234
Vogt A (1930) Lehrbuch und Atlas der Spaltlampenmikroskopie des lebenden Auges. Springer Verlag Berlin
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Stefansson, A., Müller, O. & Sundmacher, R. Non-contact specular microscopy of the normal corneal endothelium. Graefe's Arch Clin Exp Ophthalmol 218, 200–205 (1982). https://doi.org/10.1007/BF02150095
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DOI: https://doi.org/10.1007/BF02150095