Thrombin stimulates the production of immunoreactive endothelin-1 in cultured human umbilical vein endothelial cells

doi:10.1016/0026-0495(90)90157-8

Metabolism

Volume 39, Issue 10, October 1990, Pages 1003-1005

https://doi.org/10.1016/0026-0495(90)90157-8 Get rights and content

Abstract

The present study was designed to examine the effect of thrombin on endothelin production by cultured human umbilical vein endothelial cells (HUVEC) by radioimmunoassay. Cultured HUVEC released immunoreactive endothelin-1 (ir-endothelin-1) into the medium in a time-dependent manner. This release was inhibited completely by 10 μg/mL cycloheximide and is, therefore, directly related to de novo protein synthesis. Further endothelin release was stimulated by the addition of thrombin to the culture medium. Thrombin-induced ir-endothelin-1 release also was inhibited completely by 10 μg/mL cycloheximide. These results suggest that HUVEC in culture produce ir-endothelin-1 slowly, but continuously, and that this production is stimulated by thrombin.

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^☆: Supported by a Grant-in-Aid for Scientific Research (No. 6148210) from the Ministry of Education, Science and Culture, Japan.

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Preliminary reportThrombin stimulates the production of immunoreactive endothelin-1 in cultured human umbilical vein endothelial cells☆

Abstract

Eur J Pharmacol

Metabolism

Eur J Pharmacol

Biochem Biophys Res Commun

Thromb Res

Characterization of a coronary vasoconstrictor produced by cultured endothelial cells

Am J Physiol

Sustained coronary vasoconstriction provoked by a peptidergic substance released from endothelial cells in culture

J Pharmacol Exp Ther

Preliminary report
Thrombin stimulates the production of immunoreactive endothelin-1 in cultured human umbilical vein endothelial cells☆