Journal of Chromatography B: Biomedical Sciences and Applications
Determination of human urinary hyaluronic acid, chondroitin sulphate and dermatan sulphate as their unsaturated disaccharides by high-performance liquid chromatography
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Total and single species of uronic acid-bearing glycosaminoglycans in urine of newborns of 2–3 days of age for early diagnosis application
2016, Clinica Chimica ActaCitation Excerpt :UA-GAGs show important biological and pharmacological activities [2] as they are involved in several physio-pathological processes such as cell-cell interaction, cellular communication, cell signaling and in the developmental biology of organisms [4–6]. Normal human urine mainly contains CS, about 85–90% of the total, HS in a percentage of about 10–15%, and trace amounts of HA and DS (and keratan sulfate that, however, has no uronic acid inside its polysaccharide structure) [7–10]. Thanks to easy access to this biological fluid and relatively simple to process, urinary UA-GAGs may serve as biomarkers for several diseases.
A novel approach for HPLC determination of 2-cynaoacetamide using derivatization procedure with 2-hydroxyacetophenone as a new useful derivatization reagent
2016, Journal of Pharmaceutical and Biomedical AnalysisCitation Excerpt :Many reactions with 2-cynaoacetamide leading to fluorescent products are known, but the quantitative determination of 2-cynaoacetamide using separation methods with this type of derivatization reactions has not yet been published. The usefulness of 2-cyanoacetamide as a sensitive fluorogenic reagent for determination of reducing carbohydrates [5,6], catecholamines [7], uronic acids [8], aldoses [9], hexosamines [10], glycosaminoglycans (such as hyaluronic acid, chondroitin sulphate and dermatan sulphate) [11] and keratan sulfates [12] has been described. 2-Cyanoacetamide reacts with various classes carbohydrates in alkaline borate buffer at temperature of 100 °C to give highly intensive fluorescence products having various excitation and emission maxima [13].
Isolation and characterization of hyaluronic acid from marine organisms
2014, Advances in Food and Nutrition ResearchLiquid chromatography coupled to on-line post column derivatization for the determination of organic compounds: A review on instrumentation and chemistries
2013, Analytica Chimica ActaCitation Excerpt :These methods were based on a two-steps modified Hantzsch synthesis, where formaldehyde produced by the on-line alkaline decomposition of the analytes (mycotoxins) at high temperatures (step 1) reacts with acetoacetate and ammonium forming fluorescent dihydropyridine derivatives (step 2). Honda introduced this reagent for the fluorimetric detection of reducing carbohydrates; the advantage of this reagent is its compatibility with borate buffer medium that is used in carbohydrate analyzers [164]. Continuing the research on this topic, the same researcher successfully utilized 2-cyanoacetamide in a PCD mode for the electrochemical detection of reducing carbohydrates [165].
Glycosaminoglycan glycomics using mass spectrometry
2013, Molecular and Cellular ProteomicsCitation Excerpt :Lyase-generated disaccharides have a UV chromophore that allows detection with modest sensitivity. Efforts have therefore been made to improve sensitivity with fluorescence using reductive amination (19–22) or post-column fluorophore formation (23, 24). Methods that use radio (25) or optical detection systems have the advantage of simplicity but the disadvantage that standards are needed for every peak identified.