Original articlesReproducibility and clinical application of a newly developed stabilized retinal laser Doppler instrument☆
Section snippets
Methods
In the present study, the Canon Laser Blood Flowmeter model CLBF 100 (Canon, Tokyo, Japan) was used to measure the blood column diameter (D), the centerline blood velocity (V), and the blood flow (F) in major retinal vessels. The measurement of the V is based on the principle of bidirectional laser Doppler velocimetry.13 The instrument is equipped with an internal fixation target and an automatic vessel tracking system that employs a linear CCD sensor to monitor the target vessel and maintain
Results
Reproducibility, intrasession: the blood column diameters (D) of the 18 retinal arteries selected for measurement using both eyes of the 6 subjects ranged from 81 mm to 133 mm (mean, 106 mm). The time-average centerline blood velocity (Vav) ranged from 16.5 mm/s to 51.8 mm/s (mean, 30.7 mm/s). The blood flow (F) ranged from 2.9 ml/min to 17.1 ml/min (mean, 8.8 ml/min). The coefficients of variation (CV) (mean ± SD) for D, Vav, and F for the measured arteries were 5.5% ± 2.2%, 13.1% ± 6.3%, and
Discussion
The intrasession, intersession, and interobserver reproducibility of retinal blood flow measurements made using the new CLBF 100 instrument are comparable to or better than those reported for other laser-based instruments for ocular blood flow assessment. The so-called coefficient of reproducibility, which we have termed the average difference between two sets of measurements, reported for the Laser Speckle Tissue Circulation Analyzer6 was 13.0% ± 3.0% for a 24-hour interval between
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InternetAdvance publication at ajo.com Nov 26, 2002.
Doctor Feke has been a consultant for Canon, Inc.
Supported in part by a grant from the Estelle Marsh Trust, Dallas, Texas.