Repair of H2O2-induced DNA damage in bovine lens epithelial cell cultures
References (38)
- et al.
Comparison of the effects of hydrogen peroxide and X-ray irradiation on toxicity, mutation, and DNA damage/repair in mammalian cells (V-79)
Biochim. Biophys. Acta
(1981) - et al.
Effect of 3-aminobenzamide on DNA strand-break rejoining and cytotoxicity in CHO cells treated with hydrogen peroxide
Biochim. Biophys. Acta
(1986) - et al.
Protection of mammalian cells by o-phenanthroline from lethal and DNA-damaging effects produced by active oxygen species
Biochim. Biophys. Acta
(1985) A convenient program for the rapid calculation of sedimentation coefficients in linear salt or sucrose gradients
Anal. Biochem.
(1972)- et al.
Peroxide-induced effects on lens cation transport following inhibition of glutathione reductase activity in vitro
Exp. Eye Res.
(1987) - et al.
Response of cultured human lenses to H2O2
Exp. Eye Res.
(1985) - et al.
The lens: development, proteins, metabolism and cataract
- et al.
Simultaneous extraction and combined bioluminescent assay of NAD+NADH
Anal. Biochem.
(1983) - et al.
The replication of DNA in murine lymphoma cells (L5178Y). I. Rate of replication
Biochim. Biophys. Acta
(1970) ADP-ribose in DNA repair: a new component of DNA excision repair
Adv. Rad. Biol.
(1984)
Hydrogen peroxide and human cataract
Exp. Eye Res.
Does elevated glutathione protect the cell from H2O2 insult?
Exp. Eye Res.
The effect of H2O2 upon thioredoxin-enriched lens epithelial cells
J. Biol. Chem.
Radiation-induced breaks of DNA in cultured mammalian cells
Biophys. J.
Protein modification in cataract: possible oxidative mechanisms
Molecular mechanism of cataractogenesis. II. Evidence of lipid peroxidation and membrane damage
Glyceraldehyde-3-phosphate dehydrogenase from yeast
Methods Enzymol.
In vivo formation of single-strand breaks in DNA by hydrogen peroxide is mediated by the Haber-Weiss reaction
Biochim. Biophys. Acta
Oxidation of tartaric acid in presence of iron
J. Chem. Soc.
Cited by (44)
Radiation cataract
2012, Annals of the ICRPCitation Excerpt :The cellular and molecular pathways of the biological response to ionising radiation exposure in the lens have fundamental relevance and parallels to mutagenic processes in other tissues, in that radiation cataract likely arises from damaged or misrepaired DNA and subsequent errors in cell cycle control, division, and differentiation. As lens epithelial cell DNA is so easily damaged by oxidative stresses or direct photochemical action of ultraviolet light, many investigators have suggested that unrepaired DNA damage to the lens epithelium may result in cataract (Bellows, 1975; Jose, 1978; Courtois et al., 1981; Bloemendal, 1984; Rink, 1985; Spector et al., 1989; Worgul et al., 1989). This hypothesis is supported by evidence of DNA damage in human cataractous lens tissue (Worgul et al., 1991; Kleiman and Spector, 1993).
GTP binds to α-crystallin and causes a significant conformational change
2012, International Journal of Biological MacromoleculesCitation Excerpt :Binding of ATP to α-crystallin was shown to have a stabilizing effect on α-crystallin at millimolar concentrations [16]. During stressful conditions, cellular concentrations of ATP, at which α-crystallin may function, are decreased to micromolar levels [32–34]. As previously shown [14], stress increases the cell's levels of heat shock proteins; such as α-crystallin and during recovery, ATP levels begin to return to their normal range.
A sensitive enzymeless hydrogen-peroxide sensor based on epitaxially-grown Fe <inf>3</inf>O <inf>4</inf> thin film
2011, Analytica Chimica ActaCitation Excerpt :Industrial strength H2O2 is a strong oxidizer and is able to corrode eyes and skin, causing irreversible damage. H2O2 found in the eye lens and aqueous fluid, at concentrations only slightly higher than normal physiological levels, produced a significant number of DNA single-strand breaks in lens epithelial cell cultures [2] and tissue damage [3] including blindness. If consumed in excess, H2O2 can induce potentially life-threatening neurological reactions and damage to the upper gastrointestinal tract [4].
Polymorphisms of DNA repair genes XPD and XRCC1 and risk of cataract development
2007, Experimental Eye ResearchCitation Excerpt :Sidjanin et al. (1993) showed that UVA light can induce SSBs in lens epithelial cells, that the cells can repair most UVA-induced SSBs, and that UVA treatment can be toxic to the lens epithelial cells. Spector et al. (1989) suggested that lens epithelium contains enzymes that rapidly repair single-strand DNA breaks induced by H2O2 insult. Grabner and Brenner (1982) also reported unscheduled DNA repair in human lens epithelium following in vivo and in vitro ultraviolet irradiation.
Peroxide toxicity in conditioned lens epithelial cells - Evaluation of multi-defense systems
2003, Experimental Eye Research
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Recipient of a National Institutes of Health Individual National Research Service Award.
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Current address: Department of Molecular Pharmacology, Merck, Sharp and Dohme Research Laboratories, Rahway, NJ07065, U.S.A.