Preliminary reportMechanisms involved in the stimulatory effect of advanced glycation end products on growth of rat aortic smooth muscle cells
Section snippets
Culture of the cells
Rat aortic SMC were obtained from Wistar Kyoto rats (Charles River, Yokohama, Japan) using an explant method. The SMC were maintained in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO BRL, Rockville, MD) containing penicillin (100 U/mL), streptomycin (100 mg/mL), and 10% fetal bovine serum (FBS).
Galectin-3-overexpressing CHO cells were maintained in DMEM containing penicillin (100 U/mL), streptomycin (100 mg/mL), geneticin (200 μg/mL), and 10% FBS.12
Experiments were performed in compliance
Effects of glucose concentration on cell numbers
As shown in Fig 1A, incubation with 25 mmol/L glucose significantly decreased numbers of rat aortic SMC compared with 5.6 mmol/L glucose (37.6 ± 1.33 × 104 v 49.5 ± 3.00 × 104/well; P < .05; n = 8). Other concentrations of glucose did not have significant effects.
Effects of glucose concentration on tritium-thymidine uptake
High glucose concentration dose-dependently inhibited FBS-stimulated tritium-thymidine uptake by rat aortic SMC compared with 5.6 mmol/L glucose (Fig 1B). Exposure to 10.5, 15.3, 20.1, and 25 mmol/L glucose significantly and
Discussion
To clarify the mechanisms of accelerated atherosclerosis in diabetes, we examined the effects of high-glucose or high-AGE environment on growth of cultured SMC. SMC are believed to migrate within the arterial wall from the media to the intima, where their proliferation contributes to intimal thickening as a component of atherosclerosis.11 Thymidine uptake by rat aortic SMC cultured in high glucose concentrations revealed a dose-dependent decrease despite the presence of serum. No osmotic effect
Acknowledgements
We are grateful to F. Watanabe and K. Amano for their expert assistance.
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