Abstract
MOST T cells recognize antigen through the T-cell antigen receptor (TCR)αβ-CD3 complex on the T-cell surface1. A small percentage of T cells, however, do not express αβ but a second type of TCR complex designated γδ (ref. 2). Unlike αβ+ lymphocytes, γδ+ lymphocytes do not generally express CD4 or CDS molecules3–5, and the nature of antigen recognition by these cells is unknown. To study antigen recognition by γδ+ lymphocytes we raised a γδ+αβ−–CD4−CD8− line from an individual immune to PPD (purified protein derivative). This line showed a specific proliferative response to PPD and to a recombinant mycobacterial heat-shock protein (HSP) of relative molecular mass 65,000 (65K)6. The γδ+ line was shown to exhibit a major response to HSP in the presence of autologous antigen-presenting cells (APCs). Minor responses occurred, however, with APCs matched for some HLA class I or II antigens, whereas no response occurred with HLA-mismatched APCs. These findings, therefore, document the requirement of HSP-reactive γδ+ lymphocytes for histocompatible APCs.
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Haregewoin, A., Soman, G., Horn, R. et al. Human γδ+ T cells respond to mycobacterial heat-shock protein. Nature 340, 309–312 (1989). https://doi.org/10.1038/340309a0
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DOI: https://doi.org/10.1038/340309a0
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