Abstract
We studied the surface expression of activation markers IL2-R, HLA-DR and CD45-RO on peripheral T-lymphocytes in two groups of patients (n = 26) with idiopathic uveoretinitis, compared with controls. Thirteen patients were analysed by alkaline phosphatase anti-alkaline phosphatase (APAAP) immunocytochemistry, which demonstrated a significant rise in expression of HLA-DR and IL2-R surface markers. Flow cytometric analysis was performed on a further 13 patients, which confirmed a significant rise in IL2-R expression in uveitis patients. Within this group systemic activation was confined to patiepts with idiopathic retinal vasculitis. Dual flow cytometry confirmed a CD4+,IL2 - R+ ? - lymphocyte phenotype. A further 4 patients with retinal vasculitis who had been treated with cyclosporin A demonstrated a 32% reduction in IL2-R expression over a 3-month period. Analysis of CD45-RO and CD5+ cells was found to be uninformative in this study. We have demonstrated activated peripheral lymphocytes in patients predominantly with retinal vasculitis, the significance of which is discussed.
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Dick, A., Cheng, Y., Purdie, A. et al. Immunocytochemical analysis of blood lymphocytes in uveitis. Eye 6, 643–647 (1992). https://doi.org/10.1038/eye.1992.138
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DOI: https://doi.org/10.1038/eye.1992.138
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