Expression of connective tissue growth factor (CTGF) mRNA in plaques of human anterior subcapsular cataracts and membranes of posterior capsule opacification

Kerstin Wunderlich University Eye Clinic, Department of Research and Institute of Pathology of the University of Basel, Switzerland and 4Hoffmann La Roche Ltd., Basel, Switzerland

Michael Pech University Eye Clinic, Department of Research and Institute of Pathology of the University of Basel, Switzerland and 4Hoffmann La Roche Ltd., Basel, Switzerland

Alex N. Eberle University Eye Clinic, Department of Research and Institute of Pathology of the University of Basel, Switzerland and 4Hoffmann La Roche Ltd., Basel, Switzerland

Michael Mihatsch University Eye Clinic, Department of Research and Institute of Pathology of the University of Basel, Switzerland and 4Hoffmann La Roche Ltd., Basel, Switzerland

Josef Flammer University Eye Clinic, Department of Research and Institute of Pathology of the University of Basel, Switzerland and 4Hoffmann La Roche Ltd., Basel, Switzerland

Peter Meyer University Eye Clinic, Department of Research and Institute of Pathology of the University of Basel, Switzerland and 4Hoffmann La Roche Ltd., Basel, Switzerland

Abstract

Purpose. To investigate the correlation between connective tissue growth factor (CTGF) mRNA expression and immunohistochemical characteristics of anterior subcapsular cataract (ASC) formation as well as posterior capsule opacification (PCO) development (expression of type I collagen, a-smooth muscle actin and tenascin) under in vivo and under in vitro conditions in human and porcine lens epithelial cells. Methods. CTGF mRNA expression was investigated using in situ hybridization and RT-PCR. Expression of type I collagen, a-smooth muscle actin and tenascin was detected by immunohistochemical staining. Results. CTGF mRNA was expressed in human cataractous plaques of ASC and human PCO membranes, and appeared simultanously with the expression of type I collagen, a-smooth muscle actin and tenascin. Conclusion. The predominant expression of CTGF mRNA in human ASC and human PCO membranes suggests a significant role of CTGF in the pathological course of these ocular disorders.

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Expression of connective tissue growth factor (CTGF) mRNA in plaques of human anterior subcapsular cataracts and membranes of posterior capsule opacification

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Expression of connective tissue growth factor (CTGF) mRNA in plaques of human anterior subcapsular cataracts and membranes of posterior capsule opacification

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