A comparison was made of the accuracy of the Tono-Pen, Pneumatonometer, and Perkins hand-held tonometers by measuring the hydrostatically controlled intracular pressure from 10 to 50 mm Hg in human eye bank eyes. The open stopcock method was used in which the cannulated eye, the hydrostatic pressure controlling column, and a manometer were continuously open to each other. The Pneumatonometer gave accurate results at 50 mm Hg but overestimated the lower intraocular pressures; whereas, the Perkins gave satifactory results at 10 and 20 mm Hg but underestimated higher intraocular pressures. The Tono-Pen displayed the least deviation from the controlled pressure over the entire range studied. Another aspect of this study involved determining that a therapeutic soft contact lens did not have any noticeably adverse effect on the accuracy of the Pneumatonometer and Tono-Pen tonometers.