We evaluated the reproducibility of optic nerve head contour mapping by the Optic Nerve Head Analyzer with ten recordings and analyses of one eye each in ten normal volunteers and ten analyses of a single recording in three of the eyes. Reproducibility was good for cup-to-disk ratio and neural rim area. Reproducibility was poor for cup volume, which was inversely related to cup size. Instrument and operator error were low for cup-to-disk ratio and neural rim area, but high for cup volume. Subjective comparisons of contour lines from five recordings of six eyes implied good reproducibility of this measurement.