Abstract
Cross-linkage of Fc epsilon RI on human lung mast cells purified by affinity magnetic selection with monoclonal antibody YB5.B8 against c-kit (purity > 90%) expressed mRNA for multiple cytokines. There was no constitutive expression of interleukin (IL)-4 mRNA. Mast cell stimulation with anti-IgE induced IL-4 mRNA expression which appeared maximal at 2 h and waned slowly over the next 24 h. IL-5, IL-6, IL-8 and tumour necrosis factor (TNF)-alpha mRNA were constitutively expressed. Mast cell activation with anti-IgE led to an increase of IL-5 and TNF-alpha mRNA signals within 2 h and which persisted for at least 24-48 h. On the other hand, IL-6 and IL-8 mRNA expression were not affected by anti-IgE challenge.
MeSH terms
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Antibodies, Anti-Idiotypic / pharmacology
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Gene Expression Regulation / drug effects*
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Hematopoietic Cell Growth Factors / pharmacology
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Humans
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Immunoglobulin E / immunology
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Interleukin-4 / biosynthesis
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Interleukin-4 / genetics
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Interleukins / biosynthesis*
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Interleukins / genetics
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Lung / cytology
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Mast Cells / drug effects*
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Mast Cells / metabolism
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RNA, Messenger / biosynthesis*
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RNA, Messenger / genetics
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Receptor Aggregation
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Receptors, IgE / physiology*
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Signal Transduction / drug effects
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Stem Cell Factor
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Tumor Necrosis Factor-alpha / biosynthesis*
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Tumor Necrosis Factor-alpha / genetics
Substances
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Antibodies, Anti-Idiotypic
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Hematopoietic Cell Growth Factors
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Interleukins
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RNA, Messenger
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Receptors, IgE
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Stem Cell Factor
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Tumor Necrosis Factor-alpha
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Interleukin-4
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Immunoglobulin E