Production and characterization of monoclonal antibodies specific for different epitopes of human tenascin

E Balza; A Siri; M Ponassi; F Caocci; A Linnala; I Virtanen; L Zardi

doi:10.1016/0014-5793(93)80479-e

Production and characterization of monoclonal antibodies specific for different epitopes of human tenascin

FEBS Lett. 1993 Oct 11;332(1-2):39-43. doi: 10.1016/0014-5793(93)80479-e.

Authors

E Balza¹, A Siri, M Ponassi, F Caocci, A Linnala, I Virtanen, L Zardi

Affiliation

¹ Laboratory of Cell Biology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.

PMID: 7691659
DOI: 10.1016/0014-5793(93)80479-e

Abstract

We have obtained and characterized 11 monoclonal antibodies (mAbs) specific for different domains of human tenascin (TN). Five of these mAbs reacted with epitopes contained in the TN area that undergoes alternative splicing and are thus able to recognize specific TN isoforms. These mAbs are a useful tool to study the expression and distribution of TN and its different isoforms in normal and pathological tissues.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / biosynthesis*
Antibodies, Monoclonal / immunology
Antibody Specificity*
Cell Adhesion Molecules, Neuronal / immunology*
Cell Adhesion Molecules, Neuronal / metabolism
Cell Line
DNA, Complementary
Epitopes / immunology*
Extracellular Matrix / metabolism
Extracellular Matrix Proteins / immunology*
Extracellular Matrix Proteins / metabolism
Fibroblasts / immunology
Fibroblasts / metabolism
Humans
Immunohistochemistry
Melanoma / immunology
Melanoma / metabolism
Tenascin
Tumor Cells, Cultured

Substances

Antibodies, Monoclonal
Cell Adhesion Molecules, Neuronal
DNA, Complementary
Epitopes
Extracellular Matrix Proteins
Tenascin