Transfer of small YACs to E. coli as large circular plasmids

E Frengen; C Wu; P J de Jong

doi:10.1016/s1050-3862(97)00012-0

Transfer of small YACs to E. coli as large circular plasmids

Genet Anal. 1997 Jul;14(2):55-9. doi: 10.1016/s1050-3862(97)00012-0.

Authors

E Frengen¹, C Wu, P J de Jong

Affiliation

¹ Biotechnology Centre of Oslo, University of Oslo, Norway. efrengen@biotek.uio.no

PMID: 9349942
DOI: 10.1016/s1050-3862(97)00012-0

Abstract

We have designed a YAC circularization vector, pCIRC3, allowing enrichment of the YAC DNA by exonuclease digestion of the linear yeast chromosomes. Due to the presence of P1 replicon sequences in this vector, the circular YACs would replicate as PACs in Escherischia coli.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Chromosomes, Artificial, Yeast*
DNA, Circular*
Escherichia coli / genetics*
Plasmids*

Substances

DNA, Circular

Grants and funding

1R01RG01165-01/RG/CSR NIH HHS/United States