Objective To determine the calculated, anatomically correct, area of retinal non-perfusion and total area of visible retina on ultra-widefield fluorescein angiography (UWF FA) in retinal vein occlusion (RVO) and to compare the corrected measures of non-perfusion with the ischaemic index.

Methods Uncorrected UWF FA images from 32 patients with RVO were graded manually for capillary non-perfusion, which was calculated as a percentage of the total visible retina (uncorrected ischaemic index). The annotated images were converted using novel stereographic projection software to calculate precise areas of non-perfusion in mm², which was compared as a percentage of the total area of visible retina (‘corrected non-perfusion percentage’) with the ischaemic index.

Results The precise areas of peripheral non-perfusion ranged from 0 mm² to 365.4 mm² (mean 95.1 mm²), while the mean total visible retinal area was 697.0 mm². The mean corrected non-perfusion percentage was similar to the uncorrected ischaemic index (13.5% vs 14.8%, p=0.239). The corrected non-perfusion percentage correlated with uncorrected ischaemic index (R=0.978, p<0.001), but the difference in non-perfusion percentage between corrected and uncorrected metrics was as high as 14.8%.

Conclusions Using stereographic projection software, lesion areas on UWF images can be calculated in anatomically correct physical units (mm²). Eyes with RVO show large areas of peripheral retinal non-perfusion.