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Analysis of multiple genetic loci reveals MPDZ-NF1B rs1324183 as a putative genetic marker for keratoconus
  1. Yu Meng Wang1,
  2. Li Ma1,
  3. Shi Yao Lu1,
  4. Tommy Chung Yan Chan1,
  5. Jason C S Yam1,
  6. Shu Min Tang1,
  7. Ka Wai Kam1,2,
  8. Pancy O S Tam1,
  9. Clement C Tham1,
  10. Alvin L Young1,2,
  11. Vishal Jhanji1,3,
  12. Chi Pui Pang1,
  13. Li Jia Chen1,2
  1. 1 Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China
  2. 2 Department of Ophthalmology and Visual Sciences, Prince of Wales Hospital, Hong Kong, China
  3. 3 UPMC Eye Center, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
  1. Correspondence to Dr Li Jia Chen, Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong Eye Hospital, Hong Kong, China; lijia_chen{at}


Objective To investigate the associations between 16 single-nucleotide polymorphisms (SNPs) in 14 genetic loci and keratoconus in an independent Chinese cohort.

Methods This cross-sectional, case-control association study included a Chinese cohort of 133 patients with keratoconus and 371 control subjects. In a recent meta-analysis study, we identified association of 16 SNPs in 14 gene loci with keratoconus. In this study, we genotyped these 16 SNPs in all the patients and controls and analysed their association with keratoconus, its clinical severities and progression profiles. We also analysed the genotype-phenotype correlation between individual SNPs and steep keratometry, flat keratometry (Kf), average keratometry (Avg K) and best-fit sphere diameter (BFS) of the anterior and posterior corneal surface.

Results Among the 16 selected SNPs, rs1324183 in the MPDZ-NF1B locus showed a significant association with keratoconus (OR=2.22; 95% CI 1.42 to 3.45, p=4.30×10–4), especially severe keratoconus (OR=5.10, 95% CI 1.63 to 15.93, p=0.005). The rs1324183 A allele was positively associated with anterior Kf (p=0.008), anterior Avg K (p=0.017), posterior Kf (p=0.01) and negatively associated with apex pachymetry (p=0.007) and anterior BFS (p=0.023) in keratoconus. The other 15 SNPs had no significant association with keratoconus or genotype-phenotype correlations.

Conclusions This study confirmed the association of SNP rs1324183 in MPDZ-NF1B with keratoconus and revealed the association of this SNP with keratoconus severity and corneal parameters. It is thus a putative genetic marker for monitoring the progression of keratoconus to a severe form and facilitating early intervention.

  • genetics
  • cornea

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  • YMW and LM contributed equally.

  • Contributors Study concept and design: YMW, CPP, LJC. Data collection: YMW, LM, SYL, TCYC, JCSY, KWK, VJ. Analysis and interpretation of data: YMW, LM, SYL, SMT, LJC. Writing the manuscript: YMW. Critical revision of the manuscript: LM, JCSY, KWK, CCT, ALY, VJ, CPP, LJC. Administrative, technical: POST. Material support: POST, CCT, ALY, VJ, CPP, LJC. Supervision: CPP, LJC.

  • Funding This study was supported in part by a Direct Grant from the Chinese University of Hong Kong, Hong Kong (4054116).

  • Disclaimer The authors express their gratitude to all study participants.

  • Competing interests None declared.

  • Patient consent Obtained.

  • Ethics approval The Ethics Committee of the Chinese University of Hong Kong.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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