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Laboratory science
Proteomic analysis of the aqueous humour in eyes with pseudoexfoliation syndrome
  1. Amelie Botling Taube1,2,
  2. Anne Konzer3,
  3. Albert Alm1,
  4. Jonas Bergquist3
  1. 1 Department of Neuroscience, Uppsala University, Uppsala, Sweden
  2. 2 St Erik Eye Hospital, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
  3. 3 Analytical Chemistry and Neurochemistry, Department of Chemistry-BMC, Uppsala University, Uppsala, Sweden
  1. Correspondence to Professor Jonas Bergquist, Chemistry-BMC, Uppsala University, Uppsala 75124, Sweden; jonas.bergquist{at}kemi.uu.se

Abstract

Background/aims Pseudoexfoliation syndrome (PEX) is characterised by the production and accumulation of extracellular fibrillar material in the anterior segment of the eye. The pathogenesis of PEX is multifactorial with genetic factors and ageing as contributing factors. Previously, an increased concentration of beta-crystalline B2 (CRYBB2) was observed in the aqueous humour (AH) in eyes with PEX in a pooled material. Here, the protein content was examined on individual basis.

Methods During cataract surgery, AH was sampled from patients with and without PEX, 10 eyes in each group. The proteins were digested and labelled with isotopomeric dimethyl labels, separated with high-pressure liquid chromatography and analysed in an Orbitrap mass analyzer.

Results The concentration of complement factor 3, kininogen-1, antithrombin III and vitamin D-binding protein was increased in all eyes with PEX. Retinol-binding protein 3, glutathione peroxidase, calsyntenin-1 and carboxypeptidase E were decreased in eyes with PEX. Beta-crystalline B1 and CRYBB2 and gamma-crystalline D were up to eightfold upregulated in 4 of 10 in eyes with PEX.

Conclusion The results indicate that oxidative stress and inflammation are contributing factors in the formation of PEX. Knowledge about the proteome in PEX is relevant for understanding this condition.

  • aqueous humour
  • diagnostic tests/Investigation

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

https://doi.org/10.1136/bjophthalmol-2017-310416

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    Footnotes

    • Contributors ABT collected the patient samples, ABT, AK and JB analysed the data, ABT AA and JB planned and conducted the study. All authors helped writing the paper.

    • Funding This study was funded by Vetenskapsrådet (grant no: 621-2011-4423, 2015-4870).

    • Competing interests None declared.

    • Patient consent for publication Obtained.

    • Provenance and peer review Not commissioned; externally peer reviewed.

    • Data sharing statement All data are provided in the supplemental material.

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