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Optical coherence tomography angiography of perilimbal vasculature: validation of a standardised imaging algorithm
  1. Chaitali N Patel1,
  2. Anjaly K Antony2,
  3. Hasna Kommula2,
  4. Sahil Shah2,
  5. Vivek Singh1,3,
  6. Sayan Basu1,2,3
  1. 1 Prof Brien Holden Eye Research Centre, LV Prasad Eye Institute, Hyderabad, Telangana, India
  2. 2 Tej Kohli Cornea Institute, LV Prasad Eye Institute, Hyderabad, Telangana, India
  3. 3 Center for Ocular Regeneration (CORE), LV Prasad Eye Institute, Hyderabad, Telangana, India
  1. Correspondence to Dr Sayan Basu, Center for Ocular Regeneration (CORE), LV Prasad Eye Institute, Hyderabad, TG 500034, India; sayanbasu{at}


Purpose To test the reliability and accuracy of a standardised non-invasive imaging algorithm using optical coherence tomography angiography (OCTA) in detecting and quantifying pharmacologically induced changes in the perilimbal vasculature.

Methods In this prospective observational imaging study, 370 angiograms of 15 normal eyes and 10 eyes with nasal pterygiums were obtained using a commercially available OCTA system with split-spectrum amplitude-decorrelation angiography technology. Postprocessing of the images to quantify the area occupied by the blood vessels was performed using the Fiji software. The main outcome measures were reliability (intraobserver and interobserver agreement) and accuracy of the standardised algorithm in detecting and quantifying relative change (vasoconstriction and vasodilatation) in the area occupied by the blood vessels after instillation of topical vasoconstrictors.

Results The intraclass correlation coefficients for intraobserver and interobserver agreement were 0.91 and 0.88 (good to excellent), respectively. In normal eyes, significant vasoconstriction was noted at 5 min (35%–47%) after application of eye-drops, which peaked at 10 min (43%–63%) and was sustained until 20 min (35%–51%), followed by gradual recovery. Greatest effect was noted with a combination of 5% phenylephrine and 0.15% brimonidine tartrate (BT) eye-drops as compared with either one or two drops of BT alone, both at the 10 min (p=0.0058) and 20 min (p=0.0375) time points. This dose-dependent temporal trend was replicated in eyes with primary nasal pterygium (p=0.31).

Conclusions The findings suggest that OCTA can reliably and accurately detect and quantify relative changes in the perilimbal vasculature in both normal eyes and in eyes with pterygium.

  • optical coherence tomography (OCT)
  • angiography
  • imaging
  • ocular surface
  • limbus

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  • Contributors All authors contributed significantly to be eligible for authorship.

  • Funding This work was funded by the Hyderabad Eye Research Foundation, Hyderabad, India.

  • Disclaimer The sponsoring organisations had no role in the design or conduct of this research.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval The study followed the tenets of the Declaration of Helsinki and was approved prospectively by the Institutional Review Board (IRB) and the Ethics Committee, LV Prasad Eye Institute, Hyderabad, India.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement Data are available upon request.

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