Article Text

Download PDFPDF

Evaluation of meridional scans for angle closure assessment with anterior segment swept-source optical coherence tomography
  1. Natalia Porporato1,
  2. Mani Baskaran1,
  3. Shamira Perera1,
  4. Tin A Tun1,
  5. Rehena Sultana2,
  6. Marcus Tan3,
  7. Joanne HuiMin Quah4,
  8. John C Allen2,
  9. David Friedman5,
  10. Ching Yu Cheng1,
  11. Tin Aung1
  1. 1 Glaucoma, Singapore Eye Research Institute/Singapore National Eye Centre, Singapore
  2. 2 Centre for Quantitative Medicine, Duke-NUS Graduate Medical School, Singapore
  3. 3 Ophthalmology, National University Hospital, National University of Singapore, Singapore
  4. 4 Outram Polyclinic, SingHealth Polyclinics, Singapore
  5. 5 Ophthalmology, Harvard University, Cambridge, Massachusetts, USA
  1. Correspondence to Dr Tin Aung, Glaucoma, Singapore National Eye Centre, Singapore 168751, Singapore; aung.tin{at}


Background/aims As swept-source optical coherence tomography (SS-OCT) simultaneously obtains 128 meridional scans, it is important to identify which scans are playing the main role in classifying gonioscopic angle closure to simplify the analysis. We aimed to evaluate the diagnostic performance of every meridional scan in its ability to detect gonioscopic angle closure.

Methods Observational study with 2027 phakic subjects consecutively recruited from a community polyclinic. Gonioscopy and SS-OCT were performed. Gonioscopic angle closure was defined as non-visibility of the posterior trabecular meshwork in ≥180° of the angle, while SS-OCT was defined as iridotrabecular contact anterior to the scleral spur. The area under the receiver operating characteristic curve (AUC) was calculated to assess the diagnostic performance of each single scan, the sequential anticlockwise cumulative effect of those single scans and different combinations of them.

Results The AUCs of each scan ranged from 0.73 to 0.82. The single scan at 80°–260° had the highest AUC (0.82, 95% CI 0.79 to 0.84) and performed significantly better than most of the temporonasal scans (from 0° to 52° and from 153° to 179°). The superoinferior scans achieved higher AUCs compared with the temporonasal ones. When assessing the cumulative effect of adding individual scans consecutively, the peak AUC (0.80) was obtained when considering the superoinferior scans closer to 80°–85°, but no further positive cumulative effect was seen when adding the rest of the temporonasal scans of the circumference.

Conclusions In conclusion, the single SS-OCT scan at 80°–260° had the highest diagnostic performance. Our study suggests that the 360° evaluation may not translate to better clinical utility for detection of gonioscopic angle closure.

  • anterior chamber
  • glaucoma
  • imaging
View Full Text

Statistics from

Anterior segment optical coherence tomography (AS-OCT) technology has become a popular tool for assessment of angle closure in clinical settings. AS-OCT offers several advantages in comparison with the current reference standard, the gonioscopy, for angle assessment. Apart from being a recordable method, its non-contact nature may lead to a better compliance among physicians and patients. AS-OCT findings are also less dependent on observer’s training. However, it requires good image quality to be able to identify the landmarks for classification.

Time domain AS-OCT (Visante; Carl Zeiss Meditec, Dublin, California, USA) obtains one to four meridional scans of the anterior chamber angle at a time. Commonly, one vertical (corresponding to 90°–270°) and one horizontal (0°–180°) scan is assessed. It is limited by up to 30% ungradable angle images, mainly due to poor localisation of the scleral spur.1 Compared with time domain AS-OCT, the swept-source optical coherence tomography (SS-OCT, CASIA SS-1000; Tomey Corporation, Nagoya, Japan) has higher resolution and fewer ungradable images (8%).2 SS-OCT simultaneously captures 128 meridional scans (256 angle images) of the entire angle circumference in 2 s. We have shown in a previous work that SS-OCT has a good diagnostic performance for detecting gonioscopic angle closure using either 16 automatically graded circumferential scans per eye (area under the receiver operating characteristic curve (AUC) 0.83)3 or 128 manually graded scans (AUC 0.84).2 While 360° circumferential anterior segment imaging provides a more comprehensive understanding of angle status, SS-OCT is limited by the requirement to manually identify the scleral spur in a number of meridional scans, a process that is time-consuming.

The aim of this study was to evaluate the best single meridional SS-OCT scan to classify gonioscopic angle closure. This could simplify incorporation of SS-OCT into clinical practice.


Study population and recruitment

Subjects aged ≥50 years with no previous history of glaucoma, intraocular surgery or ocular trauma were consecutively recruited from a Singapore community polyclinic providing primary healthcare services. From June to September 2013, a total of 2038 consecutive subjects were eligible and screened for this study. We previously reported that 92% (1857) of the included subjects had gradable SS-OCT images; 87.8% were Chinese with a mean age of 61.8±6.7 years; and 63.5% were women.2

SS-OCT imaging

Before any contact procedure, all subjects underwent SS-OCT imaging under dark room conditions. Gentle manipulation of the upper and lower eyelid was applied by a second person for better visualisation, with special care to avoid inadvertent pressure on the globe. Patients were asked to focus on an internal fixation target, and once the patient had been optimally positioned, each eye was scanned with the three-dimensional angle analysis scan (which takes 2.4 s) using the autoalignment function. The algorithm acquires 128 consecutive meridional scans, each consisting of 512 A-scans across the anterior chamber.

All 128 meridional images (256 angles) were assessed by a single trained ophthalmologist (NP) who was masked to gonioscopy findings. We have previously reported good intraobserver and interobserver agreement for image grading using SS-OCT.4 A closed angle on an SS-OCT image was defined as contact between the iris and any part of the angle wall anterior to the scleral spur. An SS-OCT meridional scan was considered closed when either of the two angles assessed was closed. We have considered single scans instead of single angles as the software provides meridionals for analysis. Figure 1 shows the localisation of the SS-OCT meridionals scans in the right eye.

Figure 1

SS-OCT 360° analysis. Localisation of the SS-OCT meridional scans in the right eye. SS-OCT, swept-source optical coherence tomography.


Indentation gonioscopy using a Sussman 4-mirror goniolens (Ocular Instruments, Bellevue, Washington, USA) was performed in the dark by a glaucoma fellowship-trained ophthalmologist (MT) who was masked to imaging results. A weighted kappa of 0.82 was achieved in the assessment of angle grading when comparing with the readings of an ophthalmologist with subspecialty glaucoma training (TA).4 A 1 mm light beam was reduced to a narrow slit, and the vertical beam was offset horizontally for evaluating nasal and temporal angles and was maintained vertically for assessing superior and inferior angles. The examination was performed with the subject’s eye in the primary position of gaze. Care was taken to avoid light from falling on the pupil and to avoid inadvertent indentation during examination. In some cases, the gonioscopy lens was tilted slightly to allow a view of the angle over the convexity of the iris, avoiding ocular distortion. The angle in each quadrant was considered ‘closed’ if the posterior pigmented trabecular meshwork could not be seen in the primary position without indentation. An eye was classified as having angle closure if there were two or more closed quadrants. In this large sample, the distributions of gonioscopic angle closure by quadrant were 8.62% (160/1857), 4.63% (86/1857), 6.35% (118/1857) and 6.83% (127/1857), for the superior, inferior, temporal and nasal quadrants, respectively.

Statistical analysis

Only right eyes were taken for analysis to avoid intereye correlation. AUCs with 95% CIs were calculated to assess the performance of every individual SS-OCT scan, the sequential anticlockwise cumulative effect of individual scans and different scan combinations using gonioscopy as the reference standard. A χ2 test was used for comparison of AUCs. Statistical significance was set at p<0.05 and SAS V.9.4 statistical software was used.


One hundred and thirty-nine right eyes from the 1857 individuals in this study had ≥2-quadrant gonioscopic angle closure (7.5% prevalence). Figure 2 shows the diagnostic performance of each of the 128 scans (0°–180° to 179°–359°) individually. The scan 80°–260° had the highest AUC (0.82, 95% CI 0.79 to 0.84) to classify gonioscopic angle closure and performed significantly better than most of the temporonasal scans (from 0° to 180° to 52°–232° and from 153° to 333° to 179°–359°). The AUCs of individual scans ranged from 0.73 to 0.82, with superoinferior scans showing higher AUCs compared with the temporonasal ones. The scan 19°–199° had the lowest AUC (0.73, 95% CI 0.69 to 0.78).

Figure 2

Diagnostic performance of SS-OCT single scans for two-quadrant gonioscopic angle closure. Heat map (top) and dot plot (bottom) showing the AUC for the 128 individual SS-OCT scans representing the 360° of the circumference. AUC, area under the receiver operating characteristic curve; SS-OCT, swept-source optical coherence tomography.

Figure 3 shows the cumulative effect of individual scans on AUC. The peak AUC (0.80) was obtained by summing the superoinferior scans to 80°–85°; however, no additional benefit to AUC was accrued from summing temporonasal circumferential scans beyond 85° as diagnostic performance dropped substantially.

Figure 3

Cumulative effect of single SS-OCT. Cumulative diagnostic performance of the meridional SS-OCT scans consecutively added for the two-quadrant gonioscopic angle closure. AUC, area under the receiver operating characteristic curve; SS-OCT, swept-source optical coherence tomography.

The combination of the single horizontal (0°–180°)–vertical (90°–270°) scans, the combination of the diagonal scans (45°–225° and 135°–315°) or the combination of the four of them resulted in an AUC of approximately 0.80. However, they performed significantly worse than the single 80°–260°scan (p<0.001) (table 1).

Table 1

Diagnostic performance of combinations of SS-OCT single scans for two-quadrant gonioscopic angle closure


We found that the single SS-OCT scan between 80° and 260° had the highest diagnostic performance (AUC 0.82) to detect gonioscopic angle closure, performing better than the most commonly used combinations of single scans (p<0.001). This result was almost as good as manually grading 128 meridians2 when compared with gonioscopy as the reference standard. Our findings suggest that it may be unnecessary to manually evaluate all 128 SS-OCT scans to classify gonioscopic angle closure eyes based on a qualitative assessment. This novel finding may simplify using SS-OCT in clinical practice as not all scans would need to be manually marked. Further research needs to be done in order to validate these findings in other AS-OCT devices. However, AS-OCT technology remains suboptimal to perfectly discriminate angle closure based on gonioscopy, and the results did not differ much whether single or multiple scans were analysed.

We previously reported an AUC of 0.842 for the manual grading of the 128 scans (256 angles) that are imaged by SS-OCT and AUC of 0.83 for 16 meridional scans (32 angles) graded by an in-built software (iris–trabecular contact index).3 This performance resulted better than the one found in the literature for Visante in an equivalent community-based qualitative study (AUC 0.76).5 However, several studies have shown better results with Visante AS-OCT. Tun et al 6 reported an AUC of 0.90 in a tertiary eye clinic setting. Another two studies analysed Visante quantitative parameters in nasal quadrants of horizontal scans and also found higher diagnostic performance. Narayanaswamy et al 7 reported an AUC of 0.90 for the AOD750 (angle opening distance at 750 µm from the scleral spur), while Park et al 8 showed an AUC of 0.99 for AOD500 (angle opening distance at 500 µm from the scleral spur).

Interestingly, in a previous study with Visante time domain AS-OCT, we showed that including the inferior angle only (at 270°) had the best diagnostic performance (AUC 0.76) for angle closure detection.9 From an optimal quantitative assessment perspective, Blieden et al 10 reported that 16 meridional scans equally separated by 32° are enough to calculate trabecular iris circumferential volume at 750 μm, and eight meridional scans to estimate iris volume, two useful parameters to assess anterior chamber angle configuration. Whether these quantitative parameters can be accurately measured in fewer scans remains to be studied. This and our analysis are non-conservative ways of evaluating gonioscopic angle closure with AS-OCT imaging in view of optimising the diagnostic process at expenses of the variability of the condition (ie, percentage of the circumference closed).

Our study shows that scans of the superior–inferior area are the most discriminatory and play a large part in the overall diagnostic performance of SS-OCT compared with the temporonasal scans. This can be explained by the fact that the superior quadrant has the highest rates of angle closure, followed by the inferior quadrant, both by gonioscopy and AS-OCT imaging.11 In the current study population, the superior quadrant was also found to have the highest rate of angle closure.2 When comparing horizontal with vertical, SS-OCT slightly overcalls more the vertical than the horizontal meridians (vertical overcalls 35.48% (659/1857) vs horizontal overcalls 28.80% (535/1857)). Thus, the lower AUC for horizontal scans can be explained by the fact the horizontal scans and horizontal quadrants on gonioscopy had lower rates of angle closure. Similar observations with regard to the rates of angle closure in the superior quadrant have been made by other authors using gonioscopy12 13 and ultrasound biomicroscopy.14 The combination of the vertical–horizontal SS-OCT scans (0°–180° and 90°–270°) performed better than those the reported with Visante (AUC 0.80, 95% CI 0.77 to 0.83 vs AUC 0.72,9 95% CI 0.704 to 0.745), but this scan combination had still inferior performance than the single 80°–260° SS-OCT scan in the current study.

Our study has some limitations, such as the potential error in manual grading of images, reliance on a single person’s gonioscopy and use of only a Sussman lens as opposed to a Goldmann lens (which may have decreased the rate of identified angle closure due to compression on the cornea opening the angle). As this was a community-based study, there exists the potential for some inherent sampling bias as we included patients in a primary care setting in Singapore. We do not know if the scanning optimisation proposed here is generalisable to an eye clinic setting where the prevalence of angle closure would be higher and more severe disease may be present. Moreover, SS-OCT remains suboptimal to perfectly discriminate angle closure based on gonioscopy. Lastly, the test was only done on the right eyes, and we can only assume that the mirror image scan would be equally as good for the left eye.

In conclusion, our study suggests that the theoretical advantages of SS-OCT, such as 360° evaluation of the anterior chamber angle, do not translate to better clinical utility for detection of gonioscopic angle closure. The superoinferior scans and particularly the single scan at 80°–260° had the highest diagnostic performance to classify angle closure.


View Abstract


  • Contributors Conception and design of the work: NP, MB, SP, DF, CYC and TA. Acquisition, analysis or interpretation of data for the work: NP, MB, TAT, RS, MT, JHQ, JCA and TA. Drafting of the work: NP. Revising the work: NP, MB, SP, DF, CYC, TA, TAT, RS, MT, JHQ and JCA.

  • Funding This work was supported by National Medical Research Council and Biomedical Research Council, Singapore (grant number 10/1/35/19/674).

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval The study had the approval of the SingHealth ethics review board and was performed in accordance with the tenets of the Declaration of Helsinki.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement Data are available upon reasonable request.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

Linked Articles

  • At a glance
    Keith Barton James Chodosh Jost B Jonas