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Intraocular leucocyte subpopulations analysis by multiparametric flow cytometry in human uveitis
  1. Ester Carreño1,2,
  2. Cristina Serrano3,
  3. Nelida Muñoz1,
  4. Fredeswinda Romero-Bueno2,
  5. Olga Sánchez Pernaute2,
  6. Nicolas Alejandre1
  1. 1 Ophthalmology Department, Hospital Universitario Fundacion Jimenez Diaz, Madrid, Spain
  2. 2 Rheumatology Department, Hospital Universitario Rey Juan Carlos, Mostoles, Madrid, Spain
  3. 3 Clinical Immunology Department, Hospital Universitario Fundacion Jimenez Diaz, Madrid, Spain
  1. Correspondence to Dr Ester Carreño, Hospital Universitario Fundacion Jimenez Diaz, 28040 Madrid, Spain; carregnito{at}


Purpose The aim of this study was to describe the cellular infiltrate in aqueous and vitreous samples of patients with uveitis analysed by multiparametric flow cytometry.

Methods This is a retrospective analysis of aqueous and vitreous samples analysed by flow cytometry for diagnostic purposes, in cases of masquerade syndromes and infectious and non-infectious uveitis. Data collected included demographics, anatomical classification of uveitis, phenotypic diagnosis, anterior chamber cells grading, vitreous haze and time of follow-up since presentation to sample obtained.

Results Thirty-one samples (17 aqueous and 14 vitreous fluids) from 31 patients, 18 men, were analysed. The mean age at the time of sample collection was 60.23±17.03 years. The most frequent anatomical classification was panuveitis (14 of 31). T cells accounted for the main cellular component in the majority of the samples (10 of 13 aqueous samples; 7 of 14 in vitreous samples). CD4:CD8 ratios ranged from 0.21 to 16.3 in the case of aqueous samples and from 0.5 to 9.7 in the case of vitreous samples.

Discussion Flow cytometry analysis of aqueous and vitreous samples from patients with uveitis could provide insight into the pathogenesis of human uveitis and help develop accurate animal models which better mimic human disease.

  • aqueous humour
  • immunology
  • inflammation
  • vitreous

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  • Presented at Data from this study have been presented as a poster at ARVO 2019, 2 May, Vancouver, Canada.

  • Contributors Conception or design of the work: EC, CS, NA, OSP. Acquisition, analysis and interpretation of data for the work: all authors. Drafting the work and revising it critically for important intellectual content: all authors. Final approval of the version to be published: all authors.

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval The protocol of the retrospective study was approved by our institutional review board. Intraocular samples were handled in accordance with the Human Tissue Act 2004, and the research followed the tenets of the Declaration of Helsinki.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement All data relevant to the study are included in the article or uploaded as supplementary information.

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