Background As a majority of patients with choroidal melanoma do not undergo enucleation, tumour tissue for prognostic testing has to be obtained with alternate methods. Transvitreal incisional biopsies enable histological examination as well as immunohistochemical staining of BRCA1-associated protein-1 (BAP-1).
Methods Fifty-nine patients diagnosed with choroidal melanoma in transvitreal biopsies between years 2003 and 2019 were included. Twenty-one of these patients subsequently underwent enucleation. The level of nuclear expression of BAP-1 in transvitreal biopsies and enucleations was evaluated and the concordance calculated. Metastasis-free survival and HR for metastasis were analysed.
Results The mean tumour thickness and diameter at biopsy was 3.8 mm (SD 2.1) and 9.3 mm (SD 4.8), respectively. For biopsies, 37 of 59 tumours (63%) were classified as having high nuclear BAP-1 expression, and 22 (37%) as low. For enucleations, 13 of 21 tumours (62%) were classified as having high nuclear BAP-1 expression, and 8 (38%) as low. Eighty-six per cent of biopsies had an identical BAP-1 classification as the subsequent enucleation, yielding a Cohen’s kappa coefficient of 0.70. Patients with low nuclear BAP-1 expression in transvitreal biopsies had a significantly shorter metastasis-free survival (p=0.001), with a size-adjusted Cox regression HR for metastasis of 13.0 (95% CI 3.1 to 54.4, p=0.0004).
Conclusion Loss of nuclear BAP-1 expression occurred in a large proportion of the small tumours included in this study. BAP-1 immunoreactivity in transvitreal incisional biopsies of choroidal melanoma is substantially concordant with immunoreactivity in enucleated specimens and identifies patients with poor metastasis-free survival.
- diagnostic tests/investigation
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Uveal melanoma (UM) is the most common primary intraocular malignancy in adults.1 Less than 5% of patients have clinically detectable metastases at the time of primary tumour diagnosis.2 Within 15 years, however, up to 45% of patients will have died from metastatic disease even with early enucleation of the eye.3 Presumably, this is caused by subclinical dormant micrometastases, most frequently located in the liver.4 Once these start growing into clinically detectable lesions, no effective treatment is currently available and the median patient survival is only 4–15 months.5
There are several existing methods for prognostication of UM. Among these, the prognostic utility of detection of loss of expression of the tumour suppressor BRCA1-associated protein-1 (BAP-1) has recently been investigated.6 7 BAP-1 is a nuclear deubiquitinase, implicated in chromatin dynamics, the DNA damage response and regulation of the cell cycle and cell growth.8 For the expression and translocation to the nucleus, where BAP-1 can suppress tumour proliferation, one intact allele of the BAP-1 gene is necessary.9 In 80% of metastasizing UM, inactivating mutations in the gene encoding BAP-1, located on chromosome 3p21.1, have been identified.10 Further, loss of nuclear BAP-1 protein expression has been observed in tumours without a BAP-1 mutation, suggesting that the proportion of metastasizing UM with loss of nuclear BAP-1 protein expression is even higher.11
Even though BAP-1 mutations have been described to occur late in the tumour progression, it remains unclear to what extent nuclear BAP-1 expression is lost in small UM.10 Further, whereas BAP-1 immunoreactivity has been well studied on formalin-fixed paraffin embedded tissue sections from enucleated specimens and in transscleral fine needle aspiration biopsies (FNABs), BAP-1 expression in transvitreal biopsies has not been evaluated.6 9 Most patients with UM do not undergo enucleation, and compared with biopsies FNABs do not enable histological examination, do not preserve tissue architecture, retrieve a smaller number of cells and, as shown in other tumours, have less prognostic value.12 13 Further, BAP-1 status in biopsy material is not necessarily concordant with BAP-1 status in enucleated specimens, considering the difference in tumour volume represented and previous reports of intratumour heterogeneity in BAP-1 expression.14 We, therefore, see an opportunity to evaluate the expression of BAP-1 in transvitreal incisional biopsies, its prognostic utility and correlation with the expression in eyes that were subsequently enucleated.
Materials and methods
Patients and samples
All patients with a histopathologically confirmed UM originating in the choroid after a pars plana vitrectomy-assisted incisional (transvitreal) biopsy at the Department of Vitreoretinal Diseases at St. Erik Eye Hospital from 11 March 2003 to 19 March 2019 were included in this study (n=70). The procedures were performed for diagnostic purposes. In a majority of cases, tumours can be diagnosed accurately based on posterior segment examination and ultrasonography. These patients do not routinely undergo transvitreal biopsies at our institution. Preoperative informed consent was obtained from all participants. Retrospective clinical data were retrieved from digitalised clinical records. Exclusion criteria were: (1) no follow-up data available (n=8), (2) BAP-1 immunohistochemical staining failed as determined by positive controls (n=3, an unstained positive control for either biopsy or enucleation was sufficient for exclusion of the entire case) and (3) prior history of plaque brachytherapy, proton beam irradiation and/or transpupillary thermotherapy (n=0, as our previously published experience from BAP-1 staining mainly includes cohorts of untreated tumours). Fifty-nine patients remained in the study.
Transvitreal incisional biopsy technique and specimen handling
A four-port pars plana vitrectomy was performed. The biopsy site was carefully chosen usually at the location of maximum tumour thickness. A 1×1×1 mm sample of tumour tissue was incised and removed with forceps. The sample was then immediately put in 4% buffered formaldehyde and transported to the histopathological laboratory. After fixation for 24 hours, it was embedded in a block of paraffin without centrifugation, sectioning or other manipulation in order to preserve the tissue’s histological architecture. This method remained unchanged throughout the period of the study and has previously been described by Seregard et al 15 and Kvanta et al 16 in detail and is also presented here as a online supplementary video (figure 1, online supplementary video 1). Enucleated specimens are put in formaldehyde for 24 hours, after which the sclera is opened away from the tumour and the specimen fixated for another 24–72 hours depending on the size of the tumour.
To establish the diagnosis of UM versus other subretinal tumours, paraffin-embedded biopsies were cut into multiple 4 µm sections and stained with H&E and a number of immunohistochemical markers, including melanocyte markers Melan-A (Agilent Technologies, Santa Clara, California, USA), HMB45 (Agilent) and S100 (Agilent), proliferation marker Ki67 (Agilent) and epithelial markers CkAE1/3 (Agilent), ER (Novocastra, Leica Biosystems, Wetzlar, Germany), PgR (Novocastra), CdX2 (Abcam, Cambridge, UK) and TTF-1 (Novocastra). The panel of immunostains was applied to all biopsies by default to confirm the morphological impression and to rule out other malignancies. Enucleated specimens were in most cases stained with H&E and periodic acid–Schiff only, as the UM diagnosis is usually obvious without immunohistochemical confirmation. For this study, an additional 4 µm section was cut from both biopsies and enucleations, pretreated in ethylene-diamine-tetraacetic acid-buffer (EDTA) at pH 9.0 for 20 min and incubated with mouse monoclonal antibodies against BAP-1 at dilution 1:40 (clone C-4, Santa Cruz Biotechnology, Dallas, Texas, USA) and a red chromogen, and finally counterstained with haematoxylin and rinsed with deionised water. The deparaffinisation, pretreatment, primary staining, secondary staining and counterstaining steps were run in a Bond III automated stainer for immunohistochemistry/Chromogenic in situ hybridization (IHC/CISH,Leica, Wetzlar, Germany). The dilution had been gradually titrated until optimal staining was achieved, according to manual control by an ophthalmic pathologist.
The BAP-1 stained tissue sections were screened under low magnification (4 x objective). In tumour tissue obtained with transvitreal biopsy, all present tumour cells were selected for grading. In enucleated specimens, 100 cells each in three different three areas exhibiting the most intense BAP-1 staining were selected for grading. Nuclear immunoreactivity was evaluated in high magnification (20 x and/or 40 x objective). The level of BAP-1 expression was classified as low if <33% of the tumour cell nuclei were positive, and as high if ≥33% were positive (figure 2). This threshold has previously been found to correlate to risk for metastasis and gene expression class.7 The first and last author performed the classifications jointly in a masked fashion under a multiheaded microscope, blinded to patient identities and outcomes. Only nuclear staining of BAP-1 was evaluated and henceforth when referring to BAP-1 IHC, we mean the nuclear staining. External controls were used (skin and BAP-1 positive UM tissue obtained from previous enucleations). In enucleated specimens, there are also several internal controls (eg, ganglion layer of retina and/or normal choroidal/iris melanocytes and/or optic nerve nuclei).
For measurement of interspecimen concordance, the percentage of identically classified cases in transvitreal biopsies and enucleated specimens and Cohen’s kappa statistics (κ) were computed.17 For correlation with metastatic risk, BAP-1 classifications of transvitreal biopsies were evaluated with Cox proportional hazards analysis adjusted for tumour diameter and thickness, and with Kaplan-Meier metastasis-free survival. To test whether our data met the proportional hazard assumption, we built a Cox regression model with a time-dependent versus a time-independent variable (BAP-1 classification low/high). Follow-up was defined as the time in months from biopsy to the last patient encounter. Differences with a p<0.05 were considered significant, all p values being two sided. All statistical analyses were performed using IBM SPSS statistics V.25.
Of the 59 patients included, 27 were female and 32 were male. Twenty-one patients subsequently underwent enucleation. The main reason for enucleation was unfavourable tumour location for brachytherapy (eg, too close to the optic nerve) in 11, tumour size too large for brachytherapy in 4, extrascleral extension in 2, extensive retinal detachment in 2, dense vitreous bleeding in 1 and low potential for meaningful vision in 1. The mean age at transvitreal biopsy was 61 years (SD 17). Extrascleral extension was seen in 2 (3%) patients. The mean tumour thickness at biopsy was 3.8 mm (SD 2.1, minimum-maximum 1.0–10.5) and mean tumour diameter 9.3 mm (SD 4.8, minimum-maximum 2.0–25.0). Fifty-one tumours (86%) were of T-category 1 or 2, as defined by the American Joint Committee on Cancer (AJCC). No patient had undergone plaque brachytherapy, proton beam irradiation and/or transpupillary thermotherapy prior to biopsy. At enucleation, 11 tumours were of AJCC T-category 1 (52%), 4 tumours of category 2 (19%), 3 tumours of category 3 (14%) and 3 tumours of category 4 (14%). The main reason for enucleation was unfavourable tumour location (n=11). The median follow-up after biopsy was 5.3 years (IQR 5.1, minimum-maximum 0.4–14.2). Within this time period 17 patients (29%) developed metastasis and 12 patients (20%) died from metastatic disease. No recurrences in the orbit or periocular tissues were recorded. Adequate tissue for diagnosis was obtained in 59 patients (100%, table 1).
Concordance in BAP-1 classification
For the transvitreal biopsies, 37 of 59 tumours (63%) were classified as having high nuclear BAP-1 expression, and 22 (37%) as low. For the enucleated specimens, 13 of 21 tumours (62%) were classified as having high nuclear BAP-1 expression, and 8 (38%) as low. Seventeen biopsies and four enucleations had complete absence of nuclear staining. Of the 22 biopsies that were classified as having low nuclear BAP-1 expression, 12 (55%) were of AJCC T-category 1, 7 (32%) of T-category 2, 2 (9%) of T-category 3 and 1 (5%) of T-category 4 (table 2). Identical classification of nuclear BAP-1 expression was obtained in 18 of 21 (86%) patients with transvitreal biopsies and subsequent enucleations (table 3). Two patients had low nuclear BAP-1 expression in their biopsies but high nuclear BAP-1 expression in their enucleated tumours. One patient had high BAP-1 expression in the biopsy but low expression of BAP-1 in the enucleated tumour. The sensitivity and specificity for the classification in subsequent enucleations specimens was 88% and 85%, respectively, and the positive and negative predictive value 78% and 92%, respectively. The Cohen’s kappa value was 0.70, indicating substantial agreement.
Patients with low nuclear BAP-1 expression in transvitreal biopsies had a significantly increased HR for metastasis. In a multivariate Cox proportional hazards analysis with tumour diameter, tumour thickness and BAP-1 classification as covariates, the independent HR for metastasis was 13.0 (95% CI 3.1 to 54.4, p=0.0004) with a BAP-1 expression classified as low, and 1.2 (95% CI 1.1 to 1.3, p=0.005) with each increased millimetre in tumour diameter (figure 3). Tumour thickness was not independently associated with metastasis (HR 1.0 for each increased millimetre in thickness, 95% CI 0.8 to 1.3, p=0.96, table 4). The predictor satisfied the proportional hazard assumption in a Cox regression model with a time-dependent versus a time-independent treatment (p=0.36).
In Kaplan-Meier analysis, patients had a significantly shorter metastasis-free survival if their tumours had a low nuclear BAP-1 expression in transvitreal biopsies (log-rank p=0.001).
In this study, we have shown that nearly 40% of UM diagnosed with transvitreal biopsies have low BAP-1 expression. Furthermore, the BAP-1 expression in these biopsies correlates with the BAP-1 expression in enucleated specimens and identifies patients with poor metastasis-free survival.
Previous studies have found a loss of BAP-1 expression in 43% to 63% of larger tumours.7 14 17 As our cohort mainly consisted of AJCC T1 tumours, of which 55% had low BAP-1 expression, one can conclude that in a large proportion of UM, loss of BAP-1 expression occurs when the tumour is still small. This is important for understanding the metastatic process in which tumour cells with different phenotypes interact with the extracellular matrix, fibroblasts and the immune system to gain access to the systemic circulation, and for the prognostic perspective in which high-risk characteristics of UM are apparently established early on in tumour progression.14 As malignant cells likely enter the systemic circulation and establish micrometastases at a stage where the primary tumour is only a few cubic millimetres in size, factors enabling hematogeneous dissemination and metastasis should indeed be present at a very early stage.2
The majority of patients with UM will not undergo enucleation, which means that the full tumour will only be available for histopathological examination and prognostic testing in a proportion of cases. We suggest that transvitreal incisional biopsies complement FNABs in the toolbox for ophthalmologists involved in the diagnostic and prognostic work-up of UM. Consequently, BAP-1 testing can be made available for a majority of UM patients, regardless of treatment modality. Compared with FNABs, incisional biopsies have the advantage of preserved tissue architecture, a larger number of cells and a superior prognostic value.12 13
Removal of tumour tissue by transvitreal biopsy has previously been proven to be safe. In reports including some of the patients presented here, no extraocular recurrences were identified among patients that had undergone transvitreal biopsy.16 In this context, it is also noteworthy that the high concordance between biopsies and enucleations reduces concern of any major influence of spatial heterogeneity in BAP-1 expression on the results of sampling only a small fraction of the tumour for analysis.
It should be noted that although loss of BAP-1 expression usually corresponds to Monosomy 3, gene expression class and mutations in the BAP-1 gene, exceptions are not rare.11 18–20 The expression of the protein has been shown to be a strong predictor of prognosis, independent of chromosomal status11 but not gene expression class.7 18 However, to the best of our knowledge, it remains to be investigated if the level of BAP-1 protein expression or BAP-1 mutation status has stronger prognostic value.
Strengths and limitations
This study is based on real patients and tumour tissues. The combination of transvitreal biopsies and subsequent enucleated eyes allows us to directly compare the concordance of BAP-1 expression in relatively small fragments of tissue with the expression in full tumours. The access to detailed clinicopathological data about tumour and patient characteristics, as well as survival data from digitalised clinical records that are accessible regardless of where in the country the patient resides, enables robust correlation to outcome where few patients are lost to follow-up. On the other hand, there are also several limitations to this study. First, the results are based on a relatively small retrospective cohort in which a proportion of patients had short follow-up. This restricts their generalisability. Second, the proportion of patients who undergo transvitreal biopsy varies between institutions. In some ocular oncology centres, our findings will only be applicable to a small group of patients with clinically indeterminate lesions. Third, the transvitreal biopsy method is mainly appropriate for tumours posterior to the equator of the eye, while anterior tumours is better accessible with transscleral techniques. Fourth, UM prognosis depends on several factors other than BAP-1 expression. For example, we did not investigate the chromosomal status and did not perform genetic tests on the tumours included here. Fifth, the method presented herein merely predicts but currently cannot improve prognosis in UM. In our clinical experience, some patients do not want to know the results of the prognostic test when faced with this reality. It is however our opinion that the more accurate factors of importance for disease-related death we identify and define, the higher the likelihood that an effective treatment can be developed.
We analysed a Swedish cohort of 59 patients with UM, diagnosed histopathologically with transvitreal incisional biopsy. The level of BAP-1 expression was classified in the biopsy material and in 21 subsequently enucleated eyes and correlated to metastasis-free survival. A large proportion of small UM had low BAP-1 expression. The expression in biopsies and enucleations correlated substantially, and patients with low BAP-1 expression in biopsies had significantly shorter metastasis-free survival. Ideally, these findings can be confirmed in future larger and prospective cohort studies. Our results also indicate the need to further study BAP-1 expression in even smaller UM, to infer the tumour size at which loss of BAP-1 starts to occur, and to correlate it with the early seeding of micrometastases.
Contributors CH: tissue analysis, collecting data, drafting the article, revising the article. AK: surgical procedure, collecting data, revising the article. EL: tissue collection and staining. LRC: revising the article. JW: revising the article. KB: revising the article. SS: tissue analysis, revising the article. GS: conception, tissue analysis, data analysis and interpretation, drafting the article, revising the article. All authors read and approved the final manuscript.
Funding Support for this study was provided to Gustav Stålhammar from: The Royal Swedish Academy of Sciences (reference ME2019-0036); The Swedish Society of Medicine (Cronqvists stiftelse); The Swedish Eye Foundation (Ögonfonden, reference 2020-04-27); Karolinska Institutet (reference 2019–01900); Stockholm County Council (Region Stockholm).
Competing interests None declared.
Patient consent for publication Not required.
Ethics approval The study adhered to the tenets of the Declaration of Helsinki and institutional review board approval was obtained.
Provenance and peer review Not commissioned; externally peer reviewed.
Data availability statement Deidentified raw data are available from the corresponding author on reasonable request.