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Quantifying corneal endothelial cell death
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    Author Reply: Dynamics of corneal endothelial death in organ culture
    • Gilles Thuret, Ophthalmologist
    • Other Contributors:
      • Christophe Chiquet, Lydia Campos, and Philippe Gain

    Dear Editor

    We read with interest the remarks of Crowston et al. [1] on our article entitled "Value of two mortality assessment techniques for organ cultured corneal endothelium: trypan blue versus TUNEL technique".[2] We showed that the TUNEL technique revealed a far higher percentage of endothelial cells (ECs) irreversibly engaged in a cell death process than that obtained by trypan blue staining.


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    Conflict of Interest:
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