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Trypan blue staining of internal limiting membrane and epiretinal membrane during vitrectomy: visual results and histopathological findings
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  • Published on:
    Trypan blue: Authors' reply
    • Kenneth K Li, Vitreoretinal Fellow
    • Other Contributors:
      • Paul Hiscott, David Wong

    Dear Editor

    We would like to thank Dr Rodrigues and colleagues for bringing up this interesting point of what exactly trypan blue (TB) stains.

    In our study, immunohistochemistry was performed to determine the nature of cells involved in the epiretinal membranes (ERM) - not to determine the presence or absence of the ERM. Presence or absence of ERM was determined by examining routinely stained sections (H...

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    Conflict of Interest:
    None declared.
  • Published on:
    Trypan blue stains the epiretinal membrane but not the internal limiting membrane

    Dear Editor

    We read with great interest the manuscript by Li et al. about staining of the internal limiting membrane (ILM) and epiretinal membrane (ERM) with trypan blue (TB).[1] We would like to comment one aspect of this manuscript, when the authors claimed that a good staining of both the ILM and the ERM was achieved with TB. We disagree that ILM is stained by TB, and propose that TB only stains the ERM, but...

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    Conflict of Interest:
    None declared.
  • Published on:
    Authors' Reply
    • Kenneth Li, Vitreoretinal Fellow
    • Other Contributors:
      • D Wong, P Hiscott, P Stanga, C Groenewald, J McGalliard

    Dear Editor

    The authors wish to thank Gandorfer and colleagues for their interest in our paper [1] and for the kind comments and encouragement with regard to our work. Certainly, these correspondents are compiling evidence concerning the effect of indocyanine green (ICG) on the retina in both their published and unpublished studies.[2,3]

    In our report, we restricted our comments regarding retinal damage and...

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    Conflict of Interest:
    None declared.
  • Published on:
    Staining of the ILM in macular surgery
    • Arnd Gandorfer, Consultant Ophthalmologist
    • Other Contributors:
      • Christos Haritoglou, Anselm Kampik

    Dear Editor

    We read with interest the article by Dr Li and colleagues about trypan blue staining of the vitreomacular interface during vitrectomy [1]

    We congratulate the authors on their work. In particular, we appreciate their critical approach of testing trypan blue for staining of the internal limiting membrane (ILM) and epiretinal membrane (ERM) as well as their comments on potential untowards effect...

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    Conflict of Interest:
    None declared.