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Inflammatory cytokine of basal and reflex tears analysed by multicytokine assay
  1. S Sonoda,
  2. E Uchino,
  3. K Nakao,
  4. T Sakamoto
  1. Department of Ophthalmology Faculty of Medicine, Kagoshima University Graduate School of Medicine and Dental Sciences, Kagoshima, Japan
  1. Correspondence to: Taiji Sakamoto MD, Department of Ophthalmology, Faculty of Medicine, Kagoshima University Graduate School of Medicine and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan; tsakamot{at}

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Tear cytokine has a major role in various pathophysiological conditions of the ocular surface. So far, studies on tear cytokines have shown significant progress in providing an understanding of ocular surface diseases.1–3 The information that could be acquired from each subject, however, until recently has been severely hampered by limited sample volume and assay sensitivity. More importantly, it has become apparent that the relative balance between various cytokines and combinations of cytokines could be more important than absolute concentrations. Previous studies showed that the composition of basic and reflex tears was different, which made it more difficult to understand the ocular surface disorder correctly or to treat the patients suitably.4,5 Cytometric bead array (CBA) is a microparticle based flow cytometric assay that allows us to quantify multiple molecules from a very small sample.3,6,7 Using this method, we evaluated the inflammatory cytokines of basal and reflex tears from a single sample of individual eyes.


Twenty three normal volunteers (11 males and 12 females, 22–44 years of age, average 28 years) were recruited for this …

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