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Pulsed field gel electrophoresis of chromosomal bacterial DNA in the investigation of infectious endophthalmitis
  1. G B Melo1,
  2. A L Höfling-Lima1,
  3. L S Alvarenga1,
  4. J Monteiro2,
  5. A C C Pignatari2
  1. 1Department of Ophthalmology, Federal University of São Paulo, São Paulo, Brazil
  2. 2Special Laboratory for Clinical Microbiology, Division of Infectious Diseases, Federal University of Sao Paulo, São Paulo, Brazil
  1. Correspondence to: Gustavo Barreto de Melo Rua Sabará, 16, ap 113, 01239-010, São Paulo-SP, Brazil; gustavobmelo{at}

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Endogenous endophthalmitis is a rare ocular condition that accounts for only 2–6% of all cases of endophthalmitis.1 It is characterised by haematogenous spread of organisms from a remote infectious site to the eye.

Pulsed field gel electrophoresis (PFGE) might be used to determine whether the micro-organisms from the blood and the eye are the same. This technique has been used in ophthalmology in specific situations, such as severe and complex cases or unusual infectious complications (for example, endogenous endophthalmitis caused by otitis media),2 or in the confirmation of the aetiology of conjunctivitis outbreaks.3 Here we report a case of endogenous endophthalmitis caused by mitral valve infection and confirmed by PFGE.

Case report

A 51 year old man presented with pain in the left eye, fever, and a pulsatile frontal headache for 3 days. He was taking medical treatment for diabetes mellitus, systemic arterial hypertension, and renal failure, and …

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