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Letter
Agreement between two non-contact specular microscopes: Topcon SP2000P versus Rhine-Tec
  1. Gilles Thuret1,4,
  2. Nilanjana Deb-Joardar1,4,
  3. Min Zhao1,4,
  4. P Gain1,
  5. Yann Gavet2,
  6. Frederic Nguyen3,
  7. Philippe Gain4
  1. 1Laboratory ‘Biology, Engineering and Imaging of Corneal Graft’, Faculty of Medicine, University Jean Monnet, Saint- Etienne, France
  2. 2Center of Medical Engineering, Ecole Nationale Supérieure des Mines de Saint Etienne, Saint-Etienne, France
  3. 3Department of Ophthalmology, University Hospital, Saint- Etienne, France
  4. 4Laboratory ‘Cell Survival and Adherence in Cancers and Grafts’, Faculty of Medicine, University Jean Monnet, Saint Etienne, France
  1. Correspondence to: Professor P Gain Laboratory ‘Biology, Engineering and Imaging of Corneal Graft’, Faculty of Medicine, 15, Rue Ambroise Paré, 42023 Saint-Etienne, France; philippe.gain{at}univ-st-etienne.fr

https://doi.org/10.1136/bjo.2006.099374

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    Endothelial cell density (ECD) assessment with the noncontact Topcon SP2000P specular microscope is known to be reliable when the automated mode is followed by manual corrections (touched-up mode). We compared its agreement with Rhine-Tec, a new noncontact specular microscope, in 270 eyes of 160 patients, by comparing the ECD measured in the automated and touched-up modes with that of Topcon touched-up. Good agreement existed between either touched-up modes with a mean difference of only 2 cells/mm2 95% CI (−27; 23) whereas agreement with the Rhine-Tec automated mode was poor with an overestimation by a mean of 226 cells/mm2 95% CI (172; 281).

    Background

    The Topcon SP2000P non-contact specular microscope (Topcon, Tokyo, Japan) is widely used to measure corneal endothelial cell density (ECD). It uses a cell contour recognition algorithm based on contrast differences, and ECD derived from an automated delineation of cell boundaries with manual correction of inaccurately drawn cells (“touched-up” mode) has been validated.1,2 A new commercially available non-contact specular microscope Rhine-Tec (Rhine-Tec, Krefeld, Germany) determines ECD by a cell-centre method …

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    Footnotes

    • Competing interest: None.

    • Presented in part at the 18th annual meeting of the European Eye Bank Association held on 20–21 January 2006 in Venice, Italy, and 112th annual meeting of the French Society of Ophthalmology held on 6–10 May 2006 in Paris, France.