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Agreement between two non-contact specular microscopes: Topcon SP2000P versus Rhine-Tec
  1. Gilles Thuret1,4,
  2. Nilanjana Deb-Joardar1,4,
  3. Min Zhao1,4,
  4. P Gain1,
  5. Yann Gavet2,
  6. Frederic Nguyen3,
  7. Philippe Gain4
  1. 1Laboratory ‘Biology, Engineering and Imaging of Corneal Graft’, Faculty of Medicine, University Jean Monnet, Saint- Etienne, France
  2. 2Center of Medical Engineering, Ecole Nationale Supérieure des Mines de Saint Etienne, Saint-Etienne, France
  3. 3Department of Ophthalmology, University Hospital, Saint- Etienne, France
  4. 4Laboratory ‘Cell Survival and Adherence in Cancers and Grafts’, Faculty of Medicine, University Jean Monnet, Saint Etienne, France
  1. Correspondence to: Professor P Gain Laboratory ‘Biology, Engineering and Imaging of Corneal Graft’, Faculty of Medicine, 15, Rue Ambroise Paré, 42023 Saint-Etienne, France; philippe.gain{at}

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Endothelial cell density (ECD) assessment with the noncontact Topcon SP2000P specular microscope is known to be reliable when the automated mode is followed by manual corrections (touched-up mode). We compared its agreement with Rhine-Tec, a new noncontact specular microscope, in 270 eyes of 160 patients, by comparing the ECD measured in the automated and touched-up modes with that of Topcon touched-up. Good agreement existed between either touched-up modes with a mean difference of only 2 cells/mm2 95% CI (−27; 23) whereas agreement with the Rhine-Tec automated mode was poor with an overestimation by a mean of 226 cells/mm2 95% CI (172; 281).


The Topcon SP2000P non-contact specular microscope (Topcon, Tokyo, Japan) is widely used to measure corneal endothelial cell density (ECD). It uses a cell contour recognition algorithm based on contrast differences, and ECD derived from an automated delineation of cell boundaries with manual correction of inaccurately drawn cells (“touched-up” mode) has been validated.1,2 A new commercially available non-contact specular microscope Rhine-Tec (Rhine-Tec, Krefeld, Germany) determines ECD by a cell-centre method …

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