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Modifications in the surgical technique of Descemet stripping automated endothelial keratoplasty
  1. V Jhanji1,
  2. E Greenrod1,
  3. N Sharma2,
  4. R B Vajpayee1,3
  1. 1
    Royal Victorian Eye and Ear Hospital, East Melbourne, Australia
  2. 2
    Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India
  3. 3
    Centre for Eye Research Australia, University of Melbourne, Australia
  1. Professor R B Vajpayee, Centre for Eye Research Australia, University of Melbourne, 32 Gisborne Street, East Melbourne, Victoria 3002, Australia; rasikv{at}

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Descemet stripping automated endothelial keratoplasty (DSAEK) has several advantages over penetrating keratoplasty.[1, 2] Surgical outcome, however, depends upon factors like atraumatic graft insertion and reducing dislocations. During our learning curve, we tried to overcome certain problems associated with various surgical steps by developing modifications in the standard technique.

DSAEK was performed in four eyes of four patients with pseudophakic corneal oedema (n = 3) and Fuchs’ endothelial dystrophy (n = 1).

Donor-tissue preparation

An 8.0 mm donor lenticule was prepared using a microkeratome (Moria, Antony, France) using a 350 μm head.[3]

Recipient preparation

The anterior corneal surface was marked using a 9 mm marker (Moria). A peritomy was fashioned in the superior quadrant, …

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  • Competing interests: None.

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