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Intraocular lymphoma is an aggressive non-Hodgkin B cell lymphoma involving the posterior eye. Basic research on this tumour has been hindered by an inability to expand the malignant B cell population. We developed a cell culture system, in which endothelial cell monolayers were infected with adenoviral vectors encoding HIV-1 proteins, Vpu and Tat. These monolayers permitted adhesion and proliferation of CD20-positive B cells from specimens of cerebrospinal fluid obtained from patients with intracranial tumor. The system provides a method for expansion of the malignant B cell population present in small volumes of fluid that are available for research use.
Primary intraocular lymphoma (PIOL) is a diffuse large B cell lymphoma with poor prognosis due to frequent intracranial involvement;1 ocular and/or cerebral tumour is more generally described as “primary central nervous system lymphoma” (PCNSL).2 Expansion of the malignant cells has proved difficult and is a priority for studies of pathogenesis.3 AIDS-related lymphomas demonstrate aggressive growth at extranodal sites, where HIV-1 infection of endothelial cells (EC)4 may …
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