You are here

  • Home
  • Archive
  • Volume 92, Issue 2
  • Cultured human endothelial cells expressing HIV-1 Vpu and Tat support the expansion of malignant B cells from primary central nervous system lymphoma

Article Text

Article menu
Download PDFPDF
Letter
Cultured human endothelial cells expressing HIV-1 Vpu and Tat support the expansion of malignant B cells from primary central nervous system lymphoma
  1. J R Smith1,2,
  2. W W Henderson3,
  3. J T Rosenbaum1,2,4,
  4. E A Neuwelt5,
  5. A V Moses6
  1. 1
    Casey Eye Institute
  2. 2
    Departments of Cell & Developmental Biology
  3. 3
    Molecular Microbiology and Immunology
  4. 4
    Medicine
  5. 5
    Neurology
  6. 6
    Vaccine & Gene Therapy Institute, Oregon Health & Science University, Portland, OR, USA
  1. J R Smith, Biomedical Research Building, Mail Code: L467AD, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA; smithjus{at}ohsu.edu

https://doi.org/10.1136/bjo.2007.119461

Statistics from Altmetric.com

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

    Intraocular lymphoma is an aggressive non-Hodgkin B cell lymphoma involving the posterior eye. Basic research on this tumour has been hindered by an inability to expand the malignant B cell population. We developed a cell culture system, in which endothelial cell monolayers were infected with adenoviral vectors encoding HIV-1 proteins, Vpu and Tat. These monolayers permitted adhesion and proliferation of CD20-positive B cells from specimens of cerebrospinal fluid obtained from patients with intracranial tumor. The system provides a method for expansion of the malignant B cell population present in small volumes of fluid that are available for research use.

    Background

    Primary intraocular lymphoma (PIOL) is a diffuse large B cell lymphoma with poor prognosis due to frequent intracranial involvement;1 ocular and/or cerebral tumour is more generally described as “primary central nervous system lymphoma” (PCNSL).2 Expansion of the malignant cells has proved difficult and is a priority for studies of pathogenesis.3 AIDS-related lymphomas demonstrate aggressive growth at extranodal sites, where HIV-1 infection of endothelial cells (EC)4 may …

    View Full Text

    Footnotes

    • Competing interests: None.

    • Funding: Supported by NIH grants EY014909 (J R Smith) and AI063938 (A V Moses), and Research to Prevent Blindness Career Development Award (J R Smith) and Senior Scholar Award (J T Rosenbaum).