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Laboratory science
No association of CTLA-4 polymorphisms with susceptibility to Behçet disease
  1. L Du1,
  2. P Yang1,
  3. S Hou1,
  4. H Zhou2,
  5. A Kijlstra3,4
  1. 1
    The First Affiliated Hospital, Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, PR China
  2. 2
    Zhongshan Ophthalmic Center, Zhongshan, PR China
  3. 3
    Eye Research Institute Maastricht, Department of Ophthalmology, University Hospital Maastricht, Maastricht, The Netherlands
  4. 4
    Animal Sciences Group, Wageningen UR, The Netherlands
  1. Correspondence to Professor P Yang, The First Affiliated Hospital, Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing 400016, PR China; peizengycmu{at}


Background: Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) is a key negative regulator of T lymphocytes and has been shown to be associated with a number of autoimmune diseases. The present study was performed to assess the association between CTLA-4 polymorphisms and Behçet disease (BD) in Chinese patients.

Methods: Two hundred and twenty-eight BD patients and 207 controls were analysed for four single nucleotide polymorphisms (SNPs) (−1661A/G, −318C/T, +49G/A and CT60G/A) in the CTLA-4 gene by PCR-restriction fragment length polymorphism (RFLP) analysis. The association between SNP +49A/G and BD in Chinese population as well as other ethnic groups was analysed by meta-analysis.

Results: No association could be detected between CTLA-4 SNPs or haplotypes and BD. Also, no association was observed between CTLA-4 polymorphisms and BD subgroups, stratified by clinical features. A meta-analysis showed that there was no heterogeneity between studies (p = 0.60, I2 = 0%) and that CTLA-4 SNP +49 was not associated with BD (overall effect: Z = 0.26, p = 0.79).

Conclusion: This study and a meta-analysis failed to demonstrate any association between the tested CTLA-4 polymorphisms and BD.

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  • Funding This study is supported by the Fund for Key Project of Natural Science Foundation (30630064), National supporting project of PR China (2007BAI18B10), Clinical Key Project of Ministry of Health of PR China, Chongqing Key Laboratory of Ophthalmology (CSTC, 2008CA5003), Key project of Chongqing National Science Foundation (CSTC, 2009BA5037); Key project of Health Bureau (2008-1-15).

  • Competing interests None.

  • Provenance and Peer review Not commissioned; not externally peer reviewed.

  • Ethics approval Ethics approval was provided by the Ethics Committee of Zhongshan Ophthalmic Center.

  • Patient consent Obtained.