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We appreciate and the letter by Dr Shah et al regarding our article. They highlighted the usefulness of calcoflour white staining (CFW) and recommended that comparative trials between Fungiflora Y staining (FFY) and CFW be performed. As we have reported, we acknowledge the usefulness of CFW for detecting acanthamoeba cysts and agree on the need of a comparative study. The reason why we use FFY for detecting acanthamoeba cysts is that this dye is readily available in Japan, and the cost is about 1 US dollar/specimen. Furthermore, the sensitivity, technical …
Footnotes
Linked article 182972
Competing interests None.
Patient consent Obtained.
Ethics approval This study was conducted with the approval of the Institutional Review Board of Ehime University.
Provenance and peer review Not commissioned; externally peer reviewed.